Ethanol exerts differential effects on high affinity choline uptake in neuron-enriched cultures from 8-day-old chick embryo cerebral hemispheres

H. Rahman, Kendall H Lee, S. Kentroti, A. Vernadakis

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Neuronal-enriched cultures were prepared from 8-day-old chick embryo cerebral hemispheres and exposed to ethanol (50 mM) from day 4 to 8 in culture. At day 8, both control and ethanol-treated cultures were processed for [3H]choline uptake in situ. Uptake was performed on cultures containing either Na+-plus or Na+-free (Li+) HEPES buffer. Total choline uptake as well as Na+-dependent and Na+-independent choline uptake were calculated. The K(m) and V(max) were calculated using the Lineweaver-Burke analysis. Our analysis of the data revealed that ethanol-treated cultures exhibited two values for V(max), one similar to that found in control cultures and one significantly lower than controls. No differences were observed in K(m) values between control and ethanol-treated cultures. We interpret the low V(max) to represent a population of cholinergic neurons which have been arrested at an immature stage as a result of ethanol insult.

Original languageEnglish (US)
Pages (from-to)551-557
Number of pages7
JournalNeurochemical Research
Volume18
Issue number5
StatePublished - 1993
Externally publishedYes

Fingerprint

Cerebrum
Chick Embryo
Choline
Neurons
Ethanol
HEPES
Cholinergic Neurons
Cholinergic Agents
Buffers
Population

Keywords

  • choline uptake
  • Ethanol
  • neuronal cultures

ASJC Scopus subject areas

  • Biochemistry
  • Neuroscience(all)

Cite this

Ethanol exerts differential effects on high affinity choline uptake in neuron-enriched cultures from 8-day-old chick embryo cerebral hemispheres. / Rahman, H.; Lee, Kendall H; Kentroti, S.; Vernadakis, A.

In: Neurochemical Research, Vol. 18, No. 5, 1993, p. 551-557.

Research output: Contribution to journalArticle

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