Estrogen receptor expression and growth-promoting function in human choriocarcinoma cells

Norman L. Eberhardt

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Tamoxifen (1.0 μM) was found to inhibit the expression of a thymidine kinase (TK) promoter-reporter gene, lacking an estrogen response element (ERE), in transiently transfected BeWo cells, suggesting that inhibition of TK promoter activity was linked to secondary estrogen-dependent effects on BeWo cell function. Estradiol (0.05-0.45 μM) stimulated BeWo cell proliferation and increased the percentage of S-phase cells. Tamoxifen (1.35- 4.05 μM) inhibited BeWo cell growth and antagonized the stimulatory actions of 0.15 μM estradiol. Reverse transcription-polymerase chain reaction and Western analyses confirmed the presence of estrogen receptor (ER) transcripts and the 67-kD ER in BeWo cells. The BeWo cell ER binds to an ERE consensus sequence and the ER-ERE complex is supershifted by antibodies directed against the ER. We conclude that BeWo cells express a functional ER that is important for the control of BeWo cell proliferation, suggesting a potential role for estrogens in mediating placental trophoblast growth and development.

Original languageEnglish (US)
Pages (from-to)969-977
Number of pages9
JournalDNA and Cell Biology
Volume16
Issue number8
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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