Estrogen prevents destabilization of endothelial nitric oxide synthase mRNA induced by tumor necrosis factor α through estrogen receptor mediated system

Daigo Sumi, Toshio Hayashi, Muthuvel Jayachandran, Akihisa Iguchi

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

17β-estradiol up-regulates endothelial nitric oxide synthase (eNOS) expression in cultured endothelial cells. To clarify the role of mRNA stabilization in upregulation of eNOS expression, endothelial cells were incubated with actinomycin D as transcriptional inhibitor. Up to 10 hours incubation with 17β-estradiol alone did not affect significantly the stability of eNOS mRNA. As tumor necrosis factor-α (TNF-α) is associated with the progression of atherosclerosis, we examined the effect of 17β-estradiol on eNOS mRNA destabilization with TNF-α. After 10 hours co-incubation with TNF-α, relative intensity of eNOS mRNA decreased to 50% of the intensity at the start time of incubation, however, it remained significantly 1.6 times in the presence of 17β-estradiol. This inhibitory effect of 17β-estradiol was abolished by the treatment of estrogen receptor antagonist, ICI 182,780. This is the first finding that 17β-estradiol stabilizes eNOS mRNA destabilized by TNF-α through estrogen receptor mediated mechanism.

Original languageEnglish (US)
Pages (from-to)1651-1660
Number of pages10
JournalLife Sciences
Volume69
Issue number14
DOIs
StatePublished - Aug 24 2001

Keywords

  • Endothelial nitric oxide synthase
  • Estrogen
  • Stability
  • TNF-α

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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