Estrogen influences the differentiation, proliferation, and survival of early B-lineage precursors

Kay L. Medina, Andreas Strasser, Paul W. Kincade

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146 Scopus citations

Abstract

B lymphocyte production in murine bone marrow is negatively regulated by sex steroids and the aim of this study was to identify early hormone sensitive checkpoints. Estrogen (E2) treatment reduced cμ+ pre-B cells, a change that occurred concomitantly with decreased Ig gene rearrangements and rag-1 transcripts. Estrogen decreased B lineage precursors in Ig transgenic mice, demonstrating that resistant to estrogen treatment, suggestIng that life/death decisions are involved in hormonal regulation. A previously uncharacterized population of CD43-- B lineage precursors was identified in normal, Ig transgenic, and RAG(-/-) mice after estrogen treatment, revealing that down-regulation of CD43 can occur independent of Ig heavy chain expression. These cells expressed transcripts for both tdt and bcl-2, characteristics of early B-cell precursors. BrdU incorporation analysis revealed that the mitotic activity of early B-lineage cells is reduced in hormone-treated mice. We conclude that sex steroids modulate the production of B-lineage cells by influencing the differentiation, proliferation, and survival of early B-cell precursors. These findings are informative about mechanisms of hormonal regulation, as well as the significance of some differentiation-related events. (C) 2000 by The American Society of Hematology.

Original languageEnglish (US)
Pages (from-to)2059-2067
Number of pages9
JournalBlood
Volume95
Issue number6
StatePublished - Mar 15 2000

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ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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