Estimation of kinetic rate constants from surface plasmon resonance experiments

Neil D. Evans, David Lowe, David Briggs, Robert Higgins, Andrew Bentall, Simon Ball, Daniel Mitchell, Daniel Zehnder, Michael J. Chappell

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In order to characterise antibody binding characteristics it is necessary to determine reaction constants from quantitative measurements of the process. Surface plasmon resonance (SPR) provides convenient real-time measurement of the reaction that enables subsequent estimation of the reaction constants. Two models are considered that represent the binding reaction in the presence of transport effects. One of these models, the effective rate constant approximation, can be derived from the other applying a quasi-steady state assumption. Uniqueness of the reaction constants with respect to SPR measurements is considered via a structural identifiability analysis. It is shown that the effective rate constant model is unidentifiable, unless the analyte concentration is known, while the full model is structurally globally identifiable provided association and dissociation phases are considered. Both models provide comparable estimates for the unknown rate constants for a commercial anti-A monoclonal IgM experiment.

Original languageEnglish (US)
Title of host publicationUKACC International Conference on CONTROL 2010
Pages311-316
Number of pages6
Edition4
DOIs
StatePublished - Dec 1 2010
EventUKACC International Conference on CONTROL 2010 - Coventry, United Kingdom
Duration: Sep 7 2010Sep 10 2010

Publication series

NameIET Seminar Digest
Number4
Volume2010

Conference

ConferenceUKACC International Conference on CONTROL 2010
CountryUnited Kingdom
CityCoventry
Period9/7/109/10/10

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Keywords

  • Binding affinity
  • Biomedical systems
  • Parameter estimation
  • Structural identifiability
  • Surface plasmon resonance
  • Surface-volume reactions

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Evans, N. D., Lowe, D., Briggs, D., Higgins, R., Bentall, A., Ball, S., Mitchell, D., Zehnder, D., & Chappell, M. J. (2010). Estimation of kinetic rate constants from surface plasmon resonance experiments. In UKACC International Conference on CONTROL 2010 (4 ed., pp. 311-316). [0300] (IET Seminar Digest; Vol. 2010, No. 4). https://doi.org/10.1049/ic.2010.0300