Estimation of degradation rate of acetylcholine receptor by external gamma counting in vivo

G. Fumagalli, Andrew G Engel, J. Lindstrom

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We describe a novel for estimating the rate of destruction of the acetylcholine receptor (AChR) associated with the motor end-plate in the living animal. After the intramuscular injection of a nontoxic dose of α-bungarotoxin that had been monoiodinated with 125I (125I-BGT), the loss of radioactivity from the injected site is monitored by an externally positioned gamma counter. Two days after labeling, the decline of radioactivity in the injected muscle parallels the decline of end-plate specific radioactivity and can be used for the estimation of the degradation rate and half-life of end-plate AChR. Using this method, we measured AChR half-life in normal and in myasthenic rats and found a 2.5-fold decrease of AChR half-life in the myasthenic animals. This in vivo method has potential clinical applications.

Original languageEnglish (US)
Pages (from-to)758-764
Number of pages7
JournalMayo Clinic Proceedings
Volume57
Issue number12
StatePublished - 1982
Externally publishedYes

Fingerprint

Cholinergic Receptors
Radioactivity
Half-Life
Muscle Weakness
Motor Endplate
Bungarotoxins
Intramuscular Injections
Muscles

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Estimation of degradation rate of acetylcholine receptor by external gamma counting in vivo. / Fumagalli, G.; Engel, Andrew G; Lindstrom, J.

In: Mayo Clinic Proceedings, Vol. 57, No. 12, 1982, p. 758-764.

Research output: Contribution to journalArticle

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