Establishment of TUSMi008-A, an induced pluripotent stem cell (iPSC) line from a 76-year old Alzheimer's disease (AD) patient with PAXIP1 gene mutation

Ying Wang, Jing Zhang, Jinghua Hu, Gang Li, Ying Lei, Jian Zhao

Research output: Contribution to journalArticle

Abstract

A 76-year old Alzheimer's disease (AD) female patient donated her Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.

Original languageEnglish (US)
Article number101391
JournalStem Cell Research
Volume36
DOIs
StatePublished - Apr 1 2019

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Induced Pluripotent Stem Cells
Alzheimer Disease
Cell Line
Germ Layers
Mutation
Aptitude
Transgenes
Karyotype
Genes
Blood Cells
Transcription Factors
Biomarkers
Immunohistochemistry
Pharmaceutical Preparations
In Vitro Techniques

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Establishment of TUSMi008-A, an induced pluripotent stem cell (iPSC) line from a 76-year old Alzheimer's disease (AD) patient with PAXIP1 gene mutation. / Wang, Ying; Zhang, Jing; Hu, Jinghua; Li, Gang; Lei, Ying; Zhao, Jian.

In: Stem Cell Research, Vol. 36, 101391, 01.04.2019.

Research output: Contribution to journalArticle

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