Abstract
A 76-year old Alzheimer's disease (AD) female patient donated her Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.
Original language | English (US) |
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Article number | 101391 |
Journal | Stem Cell Research |
Volume | 36 |
DOIs | |
State | Published - Apr 2019 |
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology