Establishment of transgenic mouse models carrying human polymeric immunoglobulin receptor gene

Li chun Lü, Ying He, K. Lan, Xin ming Shen, Hua Huang, Pei zhou Jiang, Shuang Wang, Shou jun Xu, Kai tai Yao

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To establish transgenic mouse models carrying human polymeric immunoglobulin receptor (pIgR) gene to render the mice prone to EBV infection in normal condition, thereby to facilitate the study of the role of Epstein-Barr virus (EBV) in the pathogenesis of nasopharyngeal carcinoma. METHOD: By means of microinjection, pIgR gene under the regulation by kerotinocyte-specific promoter ED-L2 was introduced into the pronuclei of mouse zygote, which was transferred into pseudo- pregnant female mice to induce nasopharynx-specific pIgR expression in the founder mice. RESULT: Six out of the 16 founder mice (37.5%) was tested by PCR to be pIgR-positive, while Southern blotting identified only 2 positive mice. CONCLUSION: Transgenic mice harboring pIgR gene can be used potentially as a new model for studying the pothogenesis of nasopharyngeal carcinoma by EBV.

Original languageEnglish (US)
Pages (from-to)127-129
Number of pages3
JournalDi 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Volume23
Issue number2
StatePublished - Feb 2003

ASJC Scopus subject areas

  • Medicine(all)

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