Establishment of an immature mast cell line from a patient with mast cell leukemia

J. H. Butterfield, D. Weiler, G. Dewald, G. J. Gleich

Research output: Contribution to journalArticle

609 Scopus citations

Abstract

A cell line showing many characteristics of immature mast cells has been established from the peripheral blood of a patient with mast cell leukemia. Cultured cells contain low levels of histamine, are stained metachromatically by toluidine blue, and contain chloroacetate esterase, aminocaproate esterase and tryptase activities. The cells lack T and B lymphocyte, as well as myeloid cell markers, and do not possess IgE receptors. Solid tumors of metachromatically positive cells have been successfully induced and serially passed in nude mice using 5-azacytidine transformed cells. This cell line may be useful for future studies of mast cells and their constituents.

Original languageEnglish (US)
Pages (from-to)345-355
Number of pages11
JournalLeukemia Research
Volume12
Issue number4
DOIs
StatePublished - 1988

Keywords

  • Mast cells
  • chromosomal translocation
  • mast cell leukemia

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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