Establishment and Characterization of a New Human Intrahepatic Cholangiocarcinoma Cell Line LIV27

Xiwei Ding, Kais Zakharia, Catherine D. Moser, Nellie A. Campbell, Chunling Hu, Nataliya Razumilava, Roongruedee Chaiteerakij, Hassan M. Shaleh, Patricia T. Greipp, Rondell P. Graham, Xiaoping Zou, Vishal S Chandran, Lewis R. Roberts

Research output: Contribution to journalArticlepeer-review

Abstract

Cholangiocarcinoma (CCA) is a highly lethal cancer arising from the biliary tract epithelium. The cancer biology of this neoplasm is not well understood. To date, only a few CCA cell lines have been reported, which were mostly developed from Asian patients. In this study, we report and characterize a new intrahepatic CCA cell line, LIV27, derived from a surgically resected tumor in a 67-year-old Caucasian woman with primary sclerosing cholangitis (PSC). LIV27 cells grow well in collagen-coated flasks or plates with a doubling time of 57.8 h at passage 14. LIV27 cells have high tumorigenicity in nude mice and stain positive for CK7 and CK19, markers that differentiate CCA from hepatocellular carcinoma. Karyotype analysis showed that LIV27 is aneuploid. We established a single-locus short tandem repeat profile for the LIV27 cell line. This newly established cell line will be a useful model for studying the molecular pathogenesis of, and developing novel therapies for, cholangiocarcinoma.

Original languageEnglish (US)
Article number5080
JournalCancers
Volume14
Issue number20
DOIs
StatePublished - Oct 2022

Keywords

  • cell line
  • cholangiocarcinoma
  • LIV27
  • primary sclerosing cholangitis
  • short tandem repeat

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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