Essential role of the low density lipoprotein receptor-related protein in vascular smooth muscle cell migration

Yonghe Li; Wenyan Lu; Guojun Bu

doi:10.1016/S0014-5793(03)01272-9

Essential role of the low density lipoprotein receptor-related protein in vascular smooth muscle cell migration

Yonghe Li, Wenyan Lu, Guojun Bu

Neuroscience

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The low density lipoprotein receptor-related protein (LRP) is a multifunctional cell surface receptor highly expressed in human aortic smooth muscle cells. In the present study, we used the short interfering RNA (siRNA) technique to explore the role of LRP in smooth muscle cell migration. We identified an LRP-specific siRNA that selective silences LRP expression in human aortic smooth muscle cells. As a consequence, LRP-mediated ligand degradation was significantly reduced. More important, we found that platelet-derived growth factor-dependent cell migration was inhibited in cells transfected with LRP siRNA. These results demonstrate an important role of LRP in smooth muscle cell migration.

Original language	English (US)
Pages (from-to)	346-350
Number of pages	5
Journal	FEBS Letters
Volume	555
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(03)01272-9
State	Published - Dec 4 2003

Keywords

Cell migration
Ligand degradation
Low density lipoprotein receptor-related protein
Short interfering RNA
Smooth muscle cell

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(03)01272-9

Cite this

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title = "Essential role of the low density lipoprotein receptor-related protein in vascular smooth muscle cell migration",

abstract = "The low density lipoprotein receptor-related protein (LRP) is a multifunctional cell surface receptor highly expressed in human aortic smooth muscle cells. In the present study, we used the short interfering RNA (siRNA) technique to explore the role of LRP in smooth muscle cell migration. We identified an LRP-specific siRNA that selective silences LRP expression in human aortic smooth muscle cells. As a consequence, LRP-mediated ligand degradation was significantly reduced. More important, we found that platelet-derived growth factor-dependent cell migration was inhibited in cells transfected with LRP siRNA. These results demonstrate an important role of LRP in smooth muscle cell migration.",

keywords = "Cell migration, Ligand degradation, Low density lipoprotein receptor-related protein, Short interfering RNA, Smooth muscle cell",

author = "Yonghe Li and Wenyan Lu and Guojun Bu",

note = "Funding Information: This work was supported in part by a grant from the American Heart Association (0330118N) to Y.L., and a grant from the National Institutes of Health (DK61761) to G.B. G.B. is an Established Investigator of the American Heart Association.",

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T1 - Essential role of the low density lipoprotein receptor-related protein in vascular smooth muscle cell migration

AU - Li, Yonghe

AU - Lu, Wenyan

AU - Bu, Guojun

N1 - Funding Information: This work was supported in part by a grant from the American Heart Association (0330118N) to Y.L., and a grant from the National Institutes of Health (DK61761) to G.B. G.B. is an Established Investigator of the American Heart Association.

PY - 2003/12/4

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N2 - The low density lipoprotein receptor-related protein (LRP) is a multifunctional cell surface receptor highly expressed in human aortic smooth muscle cells. In the present study, we used the short interfering RNA (siRNA) technique to explore the role of LRP in smooth muscle cell migration. We identified an LRP-specific siRNA that selective silences LRP expression in human aortic smooth muscle cells. As a consequence, LRP-mediated ligand degradation was significantly reduced. More important, we found that platelet-derived growth factor-dependent cell migration was inhibited in cells transfected with LRP siRNA. These results demonstrate an important role of LRP in smooth muscle cell migration.

AB - The low density lipoprotein receptor-related protein (LRP) is a multifunctional cell surface receptor highly expressed in human aortic smooth muscle cells. In the present study, we used the short interfering RNA (siRNA) technique to explore the role of LRP in smooth muscle cell migration. We identified an LRP-specific siRNA that selective silences LRP expression in human aortic smooth muscle cells. As a consequence, LRP-mediated ligand degradation was significantly reduced. More important, we found that platelet-derived growth factor-dependent cell migration was inhibited in cells transfected with LRP siRNA. These results demonstrate an important role of LRP in smooth muscle cell migration.

KW - Cell migration

KW - Ligand degradation

KW - Low density lipoprotein receptor-related protein

KW - Short interfering RNA

KW - Smooth muscle cell

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Essential role of the low density lipoprotein receptor-related protein in vascular smooth muscle cell migration

Abstract

Keywords

ASJC Scopus subject areas

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