ESCRT-III: An endosome-associated heterooligomeric protein complex required for MVB sorting

Markus Babst, David J Katzmann, Eden J. Estepa-Sabal, Timo Meerloo, Scott D. Emr

Research output: Contribution to journalArticle

592 Citations (Scopus)

Abstract

The sorting of transmembrane proteins (e.g., cell surface receptors) into the multivesicular body (MVB) pathway to the lysosomal/vacuolar lumen requires the function of the ESCRT protein complexes. The soluble coiled-coil-containing proteins Vps2, Vps20, Vps24, and Snf7 are recruited from the cytoplasm to endosomal membranes where they oligomerize into a protein complex, ESCRT-III. ESCRT-III contains two functionally distinct subcomplexes. The Vps20-Snf7 subcomplex binds to the endosomal membrane, in part via the myristoyl group of Vps20. The Vps2-Vps24 subcomplex binds to the Vps20-Snf7 complex and thereby serves to recruit additional cofactors to this site of protein sorting. We provide evidence for a role for ESCRT-III in sorting and/or concentration of MVB cargoes.

Original languageEnglish (US)
Pages (from-to)271-282
Number of pages12
JournalDevelopmental Cell
Volume3
Issue number2
DOIs
StatePublished - Aug 2002
Externally publishedYes

Fingerprint

Endosomal Sorting Complexes Required for Transport
Multivesicular Bodies
Endosomes
Sorting
Protein Transport
Proteins
Membranes
Cell Surface Receptors
Cytoplasm

ASJC Scopus subject areas

  • Developmental Biology

Cite this

ESCRT-III : An endosome-associated heterooligomeric protein complex required for MVB sorting. / Babst, Markus; Katzmann, David J; Estepa-Sabal, Eden J.; Meerloo, Timo; Emr, Scott D.

In: Developmental Cell, Vol. 3, No. 2, 08.2002, p. 271-282.

Research output: Contribution to journalArticle

Babst, Markus ; Katzmann, David J ; Estepa-Sabal, Eden J. ; Meerloo, Timo ; Emr, Scott D. / ESCRT-III : An endosome-associated heterooligomeric protein complex required for MVB sorting. In: Developmental Cell. 2002 ; Vol. 3, No. 2. pp. 271-282.
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