Erratum

Processing of artificial peptide-DNA-conjugates by the mitochondrial intermediate peptidase (MIP) (Biol. Chem. (1999) vol 380 (961-967))

M. Seibel, C. Bachmann, J. Schmiedel, N. Wilken, F. Wilde, H. Reichmann, G. Isaya, P. Seibel, D. Pfeiler

Research output: Contribution to journalArticle

Abstract

Import of DNA from the cytoplasm into the mitochondrial matrix is an obligatory step for an in organello site-directed mutagenesis or gene therapy approach on mitochondrial DNA diseases. In this context, we have developed an artificial DNA translocation vector that is composed of the mitochondrial signal peptide of the ornithine transcarbamylase (OTC) and a DNA moiety. While this vector is capable of directing attached passenger molecules to the mitochondrial matrix, the recognition of this artificial molecule by the endogenous mitochondrial signal peptide processing machinery as well as the cleavage of the peptide plays a pivotal role in the release of the attached DNA. To study the proteolytic processing of the artificial vector, various signal peptide-DNA-conjugates were treated with purified mitochondrial intermediate peptidase. When the leader peptide is directly linked to the DNA moiety without an intervening spacer, MIP processing is prevented. Cleavage of the peptide can be restored, however, when the first ten amino acid residues of the mature part of OTC are appended at the carboxy-terminal end of the signal peptide. Our results show that artificial peptide-DNA-conjugates are recognized by the mitochondrial proteolytic machinery, and therefore an interference of the peptide with the DNA function can be excluded.

Original languageEnglish (US)
Pages (from-to)633
Number of pages1
JournalBiological Chemistry
Volume381
Issue number7
StatePublished - 2000
Externally publishedYes

Fingerprint

Protein Sorting Signals
Peptides
DNA
Processing
Ornithine Carbamoyltransferase
Machinery
Gene therapy
Mitochondrial Diseases
Mutagenesis
Molecules
mitochondrial intermediate peptidase
Site-Directed Mutagenesis
Mitochondrial DNA
Genetic Therapy
Cytoplasm
Amino Acids

Keywords

  • Gene therapy
  • Mitochondria
  • Mitochondrial processing peptidase
  • Ornithine transcarbamylase
  • Site-directed mutagenesis
  • Transformation vector

ASJC Scopus subject areas

  • Biochemistry

Cite this

Seibel, M., Bachmann, C., Schmiedel, J., Wilken, N., Wilde, F., Reichmann, H., ... Pfeiler, D. (2000). Erratum: Processing of artificial peptide-DNA-conjugates by the mitochondrial intermediate peptidase (MIP) (Biol. Chem. (1999) vol 380 (961-967)). Biological Chemistry, 381(7), 633.

Erratum : Processing of artificial peptide-DNA-conjugates by the mitochondrial intermediate peptidase (MIP) (Biol. Chem. (1999) vol 380 (961-967)). / Seibel, M.; Bachmann, C.; Schmiedel, J.; Wilken, N.; Wilde, F.; Reichmann, H.; Isaya, G.; Seibel, P.; Pfeiler, D.

In: Biological Chemistry, Vol. 381, No. 7, 2000, p. 633.

Research output: Contribution to journalArticle

Seibel, M, Bachmann, C, Schmiedel, J, Wilken, N, Wilde, F, Reichmann, H, Isaya, G, Seibel, P & Pfeiler, D 2000, 'Erratum: Processing of artificial peptide-DNA-conjugates by the mitochondrial intermediate peptidase (MIP) (Biol. Chem. (1999) vol 380 (961-967))', Biological Chemistry, vol. 381, no. 7, pp. 633.
Seibel, M. ; Bachmann, C. ; Schmiedel, J. ; Wilken, N. ; Wilde, F. ; Reichmann, H. ; Isaya, G. ; Seibel, P. ; Pfeiler, D. / Erratum : Processing of artificial peptide-DNA-conjugates by the mitochondrial intermediate peptidase (MIP) (Biol. Chem. (1999) vol 380 (961-967)). In: Biological Chemistry. 2000 ; Vol. 381, No. 7. pp. 633.
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