Erichrome stain for myelin on osmicated tissue embedded in glycol methacrylate plastic

Mabel Pierce, Moses Rodriguez

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

This paper describes the modification of the Lieb erichrome technique for staining myelin sheaths' embedded in glycol methacrylate plastic. The technique permits excellent morphologic detail of myelinated and demyelinated axons in the central nervous system and the characterization of pathologic abnormalities in the spinal cord of mice persistently infected with a demyelinating strain of Theiler's virus. It is a simple, rapid technique that allows 25–30 spinal cord cross-sections to be embedded, sectioned and stained at one time. (The J Histotechnol 12:35, 1989).

Original languageEnglish (US)
Pages (from-to)35-36
Number of pages2
JournalJournal of Histotechnology
Volume12
Issue number1
DOIs
StatePublished - Mar 1989

Keywords

  • Demyelination
  • Myelin
  • Theiler’s virus

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Medical Laboratory Technology

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