Erichrome stain for myelin on osmicated tissue embedded in glycol methacrylate plastic

M. Pierce, M. Rodriquez

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

This paper describes the modification of the Lieb erichrome technique for staining myelin sheaths embedded in glycol methacrylate plastic. The technique permits excellent morphologic detail of myelinated and demyelinated axons in the central nervous system and the characterization of pathologic abnormalities in the spinal cord of mice persistently infected with a demyelinating strain of Theiler's virus. It is a simple, rapid technique that allows 25-30 spinal cord cross-sections to be embedded, sectioned and stained at one time.

Original languageEnglish (US)
Pages (from-to)35-36
Number of pages2
JournalJournal of Histotechnology
Volume12
Issue number1
StatePublished - 1989

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Myelin Sheath
Plastics
Spinal Cord
Coloring Agents
Theilovirus
Axons
Central Nervous System
Staining and Labeling
hydroxyethyl methacrylate

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Erichrome stain for myelin on osmicated tissue embedded in glycol methacrylate plastic. / Pierce, M.; Rodriquez, M.

In: Journal of Histotechnology, Vol. 12, No. 1, 1989, p. 35-36.

Research output: Contribution to journalArticle

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