Epstein-Barr Virus (EBV) load determination using real-time quantitative polymerase chain reaction

Hongxin Fan, Ryan S. Robetorye

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) infects virtually the entire human population and infection persists throughout the lifetime of its host. EBV has been associated with the development of a wide variety of neoplasms, including lymphoma, carcinoma, and sarcoma. In addition, EBV-associated lymphoproliferative disorders are particularly prevalent in immunosuppressed individuals, including AIDS patients, transplant recipients, and patients with congenital immunodeficiencies. In recent years, EBV viral load assessment has been extensively implemented in clinical practice for the diagnosis and monitoring of EBV-associated malignancies and lymphoproliferative disorders. The real-time quantitative polymerase chain reaction (RQ-PCR) has become the method of choice for quantification of specific EBV nucleic acid sequences. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input nucleic acid, and is relatively simple to perform. These characteristics have made it the method of choice for EBV viral load determination. This chapter describes the use of a laboratory-developed RQ-PCR EBV viral load assay for the detection of EBV DNA in cell-free plasma and cerebrospinal fluid samples.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages231-243
Number of pages13
Volume999
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume999
ISSN (Print)10643745

Fingerprint

Human Herpesvirus 4
Real-Time Polymerase Chain Reaction
Viral Load
Lymphoproliferative Disorders
Nucleic Acids
Plasma Cells
Sarcoma
Cerebrospinal Fluid
Lymphoma
Neoplasms
Acquired Immunodeficiency Syndrome
Carcinoma
DNA
Infection
Population

Keywords

  • Epstein-Barr virus
  • Quantitative real-time PCR
  • Viral load

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Fan, H., & Robetorye, R. S. (2013). Epstein-Barr Virus (EBV) load determination using real-time quantitative polymerase chain reaction. In Methods in Molecular Biology (Vol. 999, pp. 231-243). (Methods in Molecular Biology; Vol. 999). https://doi.org/10.1007/978-1-62703-357-2-17

Epstein-Barr Virus (EBV) load determination using real-time quantitative polymerase chain reaction. / Fan, Hongxin; Robetorye, Ryan S.

Methods in Molecular Biology. Vol. 999 2013. p. 231-243 (Methods in Molecular Biology; Vol. 999).

Research output: Chapter in Book/Report/Conference proceedingChapter

Fan, H & Robetorye, RS 2013, Epstein-Barr Virus (EBV) load determination using real-time quantitative polymerase chain reaction. in Methods in Molecular Biology. vol. 999, Methods in Molecular Biology, vol. 999, pp. 231-243. https://doi.org/10.1007/978-1-62703-357-2-17
Fan H, Robetorye RS. Epstein-Barr Virus (EBV) load determination using real-time quantitative polymerase chain reaction. In Methods in Molecular Biology. Vol. 999. 2013. p. 231-243. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-357-2-17
Fan, Hongxin ; Robetorye, Ryan S. / Epstein-Barr Virus (EBV) load determination using real-time quantitative polymerase chain reaction. Methods in Molecular Biology. Vol. 999 2013. pp. 231-243 (Methods in Molecular Biology).
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