Epstein-Barr virus (EBV) in endemic Burkitt's lymphoma: Molecular analysis of primary tumor tissue

Qian Tao, Keith D Robertson, Angela Manns, Allan Hildesheim, Richard F. Ambinder

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Many aspects of Epstein-Barr virus (EBV) and tumor biology have been studied in Burkitt's lymphoma (BL)-derived cell lines. However, in tissue culture, patterns of gene expression and C promoter-G (CpG) methylation often change and viral strain selection may occur. In this report, 10 cases of snapfrozen endemic BL tumors are characterized in terms of viral gene expression, promoter usage, methylation, and viral strain. EBNA1 and BamHI-A rightward transcripts (BART) were detected in 7 of 7 and LMP2A transcripts in 5 of 7 tumors with well-preserved RNA. Transcripts for the other EBNAs and for LMP1 were not detected in any tumor. These tumors differ from BL cell lines in that they lack a variety of lytic cycle transcripts. This pattern of viral gene expression in endemic BL is similar to that reported in peripheral blood mononuclear cells (PBMCs) from healthy EBV-seropositive individuals. EBNA1 transcripts originated from the Q promoter (Qp) but not C, W, or F promoters that drive transcription of EBNA1 in other circumstances. Whereas Cp has been previously shown to be entirely CpG methylated in BL, bisulfite genomic sequencing showed virtually no methylation in Qp. Type-A EBV was detected in 6 of 10 and type B in 4 of 10 cases. A previously reported 30bp deletion variant in the carboxyl terminal of LMP1 gene was detected in 5 of 10 cases. The association with both A and B strains contrasts with EBV- associated Hodgkin's disease, nasopharyngeal carcinoma, and post-transplant lymphoproliferative disease, which are much more consistently associated with A strain virus.

Original languageEnglish (US)
Pages (from-to)1373-1381
Number of pages9
JournalBlood
Volume91
Issue number4
StatePublished - Feb 15 1998
Externally publishedYes

Fingerprint

Burkitt Lymphoma
Human Herpesvirus 4
Viruses
Tumors
Methylation
Tissue
Gene expression
Neoplasms
Viral Genes
Gene Expression
Cells
Tissue culture
Transplants
Cell Line
Transcription
Hodgkin Disease
Blood
Genes
Association reactions
RNA

ASJC Scopus subject areas

  • Hematology

Cite this

Tao, Q., Robertson, K. D., Manns, A., Hildesheim, A., & Ambinder, R. F. (1998). Epstein-Barr virus (EBV) in endemic Burkitt's lymphoma: Molecular analysis of primary tumor tissue. Blood, 91(4), 1373-1381.

Epstein-Barr virus (EBV) in endemic Burkitt's lymphoma : Molecular analysis of primary tumor tissue. / Tao, Qian; Robertson, Keith D; Manns, Angela; Hildesheim, Allan; Ambinder, Richard F.

In: Blood, Vol. 91, No. 4, 15.02.1998, p. 1373-1381.

Research output: Contribution to journalArticle

Tao, Q, Robertson, KD, Manns, A, Hildesheim, A & Ambinder, RF 1998, 'Epstein-Barr virus (EBV) in endemic Burkitt's lymphoma: Molecular analysis of primary tumor tissue', Blood, vol. 91, no. 4, pp. 1373-1381.
Tao, Qian ; Robertson, Keith D ; Manns, Angela ; Hildesheim, Allan ; Ambinder, Richard F. / Epstein-Barr virus (EBV) in endemic Burkitt's lymphoma : Molecular analysis of primary tumor tissue. In: Blood. 1998 ; Vol. 91, No. 4. pp. 1373-1381.
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