Epstein-Barr virus DNA is not increased in tonsillar carcinoma

Nissim Khabie, Athanasia Savva, Jan Kasperbauer, Renee McGovern, Bobbie Gostout, Scott E. Strome

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) is a known oncogenic virus associated with a wide variety of cancers, including nasopharyngeal carcinoma. Waldeyer's ring, a collection of lymphoid tissues, includes the nasopharynx, pharyngeal, and lingual tonsils. To determine if EBV plays a causative role in carcinomas arising from other tissues in Waldeyer's ring, we examined pharyngeal tonsillar carcinomas for evidence of EBV infection. As previously reported, DNA was extracted from 53 consecutive tonsil cancers, as well as from age- and gender-matched non-cancerous tonsillectomy specimens. Three different sets of primers for discrete exons of EBV were then used to determine if active or latent EBV infection was expressed in the extracted DNA using the polymerase chain reaction (PCR). All positive bands were then sequenced to confirm the presence of amplified EBV fragments. None of the samples showed evidence for active EBV infection. In primers demonstrating latent infection, 1 of 53 (1.9%) of tumors were positive, versus 6 of 53 (11.3%) of the controls. These results indicate that EBV expression is not increased in DNA from tonsil cancers and that EBV infection does not have a causal relationship with tonsil cancer.

Original languageEnglish (US)
Pages (from-to)811-814
Number of pages4
JournalLaryngoscope
Volume111
Issue number5
DOIs
StatePublished - Jan 1 2001

Fingerprint

Human Herpesvirus 4
Tonsillar Neoplasms
Epstein-Barr Virus Infections
Carcinoma
DNA
Adenoids
Oncogenic Viruses
Tonsillectomy
Nasopharynx
Lymphoid Tissue
DNA-Directed DNA Polymerase
Tongue
Exons
Neoplasms
Polymerase Chain Reaction
Infection

Keywords

  • Epstein-Barr virus
  • Polymerase chain reaction
  • Squamous cell carcinoma of the tonsils

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Khabie, N., Savva, A., Kasperbauer, J., McGovern, R., Gostout, B., & Strome, S. E. (2001). Epstein-Barr virus DNA is not increased in tonsillar carcinoma. Laryngoscope, 111(5), 811-814. https://doi.org/10.1097/00005537-200105000-00011

Epstein-Barr virus DNA is not increased in tonsillar carcinoma. / Khabie, Nissim; Savva, Athanasia; Kasperbauer, Jan; McGovern, Renee; Gostout, Bobbie; Strome, Scott E.

In: Laryngoscope, Vol. 111, No. 5, 01.01.2001, p. 811-814.

Research output: Contribution to journalArticle

Khabie, N, Savva, A, Kasperbauer, J, McGovern, R, Gostout, B & Strome, SE 2001, 'Epstein-Barr virus DNA is not increased in tonsillar carcinoma', Laryngoscope, vol. 111, no. 5, pp. 811-814. https://doi.org/10.1097/00005537-200105000-00011
Khabie, Nissim ; Savva, Athanasia ; Kasperbauer, Jan ; McGovern, Renee ; Gostout, Bobbie ; Strome, Scott E. / Epstein-Barr virus DNA is not increased in tonsillar carcinoma. In: Laryngoscope. 2001 ; Vol. 111, No. 5. pp. 811-814.
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