Epitopes of the human erythrocyte Ca2+-Mg2+ ATPase pump in human osteoblast-like cell plasma membranes

J. L. Borke, E. F. Eriksen, J. Minami, P. Keeting, K. G. Mann, J. T. Penniston, B. L. Riggs, Rajiv Kumar

Research output: Contribution to journalArticle

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Abstract

Human osteoblast-like cells were examined for the presence of the Ca2+-Mg2+ ATPase pump. The osteoblast-like cells had characteristic features of the oesteoblast phenotype, including the presence of osteonectin, bone GLA protein, and type I collagen. The cells were able to mineralize matrix, their production of cAMP increased in response to PTH, and their alkaline phosphatase activity increased in response to 1,25-dihydroxyvitamin D3. Immunocytochemical staining of the osteoblast-like cells with a monoclonal antibody against human red cell Ca2+-Mg2+ ATPase demonstrated the presence of an epitope of the Ca2+-Mg2+ ATPase in these cells; staining of paraffin-embedded osteoblast-like cell sections demonstrated anti-Ca2+-Mg2+ ATPase staining only in cell plasma membranes. Western blot analysis of osteoblast-like cell homogenates showed that the monoclonal antibody to human erythrocyte Ca2+-Mg2+ ATPase bound to a major band at 140,000 mol wt, similar to the mol wt of known plasma membrane Ca2+-Mg2+ ATPases. The presence in the osteoblast-like cells of a Ca2+-Mg2+ ATPase similar to the human red cell calcium pump suggests that this enzyme may play a role in osteoblast intracellular calcium homeostasis.

Original languageEnglish (US)
Pages (from-to)1299-1304
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume67
Issue number6
StatePublished - 1988

Fingerprint

Ca(2+) Mg(2+)-ATPase
Osteoblasts
Cell membranes
Epitopes
Erythrocytes
Cell Membrane
Pumps
Monoclonal Antibodies
Cells
Osteonectin
Staining and Labeling
Calcium
Calcitriol
Collagen Type I
Paraffin
Alkaline Phosphatase
Bone
Homeostasis
Western Blotting
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Borke, J. L., Eriksen, E. F., Minami, J., Keeting, P., Mann, K. G., Penniston, J. T., ... Kumar, R. (1988). Epitopes of the human erythrocyte Ca2+-Mg2+ ATPase pump in human osteoblast-like cell plasma membranes. Journal of Clinical Endocrinology and Metabolism, 67(6), 1299-1304.

Epitopes of the human erythrocyte Ca2+-Mg2+ ATPase pump in human osteoblast-like cell plasma membranes. / Borke, J. L.; Eriksen, E. F.; Minami, J.; Keeting, P.; Mann, K. G.; Penniston, J. T.; Riggs, B. L.; Kumar, Rajiv.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 67, No. 6, 1988, p. 1299-1304.

Research output: Contribution to journalArticle

Borke, JL, Eriksen, EF, Minami, J, Keeting, P, Mann, KG, Penniston, JT, Riggs, BL & Kumar, R 1988, 'Epitopes of the human erythrocyte Ca2+-Mg2+ ATPase pump in human osteoblast-like cell plasma membranes', Journal of Clinical Endocrinology and Metabolism, vol. 67, no. 6, pp. 1299-1304.
Borke, J. L. ; Eriksen, E. F. ; Minami, J. ; Keeting, P. ; Mann, K. G. ; Penniston, J. T. ; Riggs, B. L. ; Kumar, Rajiv. / Epitopes of the human erythrocyte Ca2+-Mg2+ ATPase pump in human osteoblast-like cell plasma membranes. In: Journal of Clinical Endocrinology and Metabolism. 1988 ; Vol. 67, No. 6. pp. 1299-1304.
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