Epitope Dampening Monotypic Measles Virus Hemagglutinin Glycoprotein Results in Resistance to Cocktail of Monoclonal Antibodies

Patrycja J. Lech, Gregory J. Tobin, Ruth Bushnell, Emily Gutschenritter, Linh D. Pham, Rebecca Nace, Els Verhoeyen, François Loïc Cosset, Claude P. Muller, Stephen J Russell, Peter L. Nara

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The measles virus (MV) is serologically monotypic. Life-long immunity is conferred by a single attack of measles or following vaccination with the MV vaccine. This is contrary to viruses such as influenza, which readily develop resistance to the immune system and recur. A better understanding of factors that restrain MV to one serotype may allow us to predict if MV will remain monotypic in the future and influence the design of novel MV vaccines and therapeutics. MV hemagglutinin (H) glycoprotein, binds to cellular receptors and subsequently triggers the fusion (F) glycoprotein to fuse the virus into the cell. H is also the major target for neutralizing antibodies. To explore if MV remains monotypic due to a lack of plasticity of the H glycoprotein, we used the technology of Immune Dampening to generate viruses with rationally designed N-linked glycosylation sites and mutations in different epitopes and screened for viruses that escaped monoclonal antibodies (mAbs). We then combined rationally designed mutations with naturally selected mutations to generate a virus resistant to a cocktail of neutralizing mAbs targeting four different epitopes simultaneously. Two epitopes were protected by engineered N-linked glycosylations and two epitopes acquired escape mutations via two consecutive rounds of artificial selection in the presence of mAbs. Three of these epitopes were targeted by mAbs known to interfere with receptor binding. Results demonstrate that, within the epitopes analyzed, H can tolerate mutations in different residues and additional N-linked glycosylations to escape mAbs. Understanding the degree of change that H can tolerate is important as we follow its evolution in a host whose immunity is vaccine induced by genotype A strains instead of multiple genetically distinct wild-type MVs.

Original languageEnglish (US)
Article numbere52306
JournalPLoS One
Volume8
Issue number1
DOIs
StatePublished - Jan 3 2013

Fingerprint

Measles virus
Hemagglutinins
hemagglutinins
Viruses
epitopes
glycoproteins
Epitopes
monoclonal antibodies
Glycoproteins
Monoclonal Antibodies
mutation
glycosylation
viruses
Mutation
Glycosylation
Measles Vaccine
vaccines
Neutralizing Antibodies
neutralizing antibodies
Immunity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lech, P. J., Tobin, G. J., Bushnell, R., Gutschenritter, E., Pham, L. D., Nace, R., ... Nara, P. L. (2013). Epitope Dampening Monotypic Measles Virus Hemagglutinin Glycoprotein Results in Resistance to Cocktail of Monoclonal Antibodies. PLoS One, 8(1), [e52306]. https://doi.org/10.1371/journal.pone.0052306

Epitope Dampening Monotypic Measles Virus Hemagglutinin Glycoprotein Results in Resistance to Cocktail of Monoclonal Antibodies. / Lech, Patrycja J.; Tobin, Gregory J.; Bushnell, Ruth; Gutschenritter, Emily; Pham, Linh D.; Nace, Rebecca; Verhoeyen, Els; Cosset, François Loïc; Muller, Claude P.; Russell, Stephen J; Nara, Peter L.

In: PLoS One, Vol. 8, No. 1, e52306, 03.01.2013.

Research output: Contribution to journalArticle

Lech, PJ, Tobin, GJ, Bushnell, R, Gutschenritter, E, Pham, LD, Nace, R, Verhoeyen, E, Cosset, FL, Muller, CP, Russell, SJ & Nara, PL 2013, 'Epitope Dampening Monotypic Measles Virus Hemagglutinin Glycoprotein Results in Resistance to Cocktail of Monoclonal Antibodies', PLoS One, vol. 8, no. 1, e52306. https://doi.org/10.1371/journal.pone.0052306
Lech, Patrycja J. ; Tobin, Gregory J. ; Bushnell, Ruth ; Gutschenritter, Emily ; Pham, Linh D. ; Nace, Rebecca ; Verhoeyen, Els ; Cosset, François Loïc ; Muller, Claude P. ; Russell, Stephen J ; Nara, Peter L. / Epitope Dampening Monotypic Measles Virus Hemagglutinin Glycoprotein Results in Resistance to Cocktail of Monoclonal Antibodies. In: PLoS One. 2013 ; Vol. 8, No. 1.
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