Epigenetic regulation of Myc on retinoic acid receptor beta and PDLIM4 in RWPE1 cells

Meilan He, Donkena Krishna Vanaja, R. Jeffrey Karnes, Charles Y.F. Young

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

BACKGROUND. Hypermethylation of CpG islands is a common epigenetic alteration associated with cancer. Tumor suppressor genes retinoic acid receptor beta (RARβ) and PDLIM4 are hypermethylated and silenced in prostate cancer (PCa) tissues and PCa cell lines compared to normal prostate cells. METHODS. In this study, a benign prostate epithelial cell line RWPE1 was used as a model to study the epigenetic regulation of Myc on the RARβ and PDLIM4 promoters. Forced Myc overexpression inhibited the RARβ and PDLIM4 expression. RESULTS. Pyrosequencing study showed that Myc overexpression increased methylation in several CpG sites of both promoters. A DNA methylation inhibitor 5-aza-20-deoxycytidine reversed the epigenetic alteration effect of Myc on both RARβ and PDLIM4. CONCLUSION. The epigenetic regulation of Myc may be related to its up-regulation of the DNA methyltransferase DNMT3a and DNMT3b.

Original languageEnglish (US)
Pages (from-to)1643-1650
Number of pages8
JournalProstate
Volume69
Issue number15
DOIs
StatePublished - Nov 1 2009

Keywords

  • Epigenetic
  • Myc
  • PDLIM4
  • Prostate cancer
  • RARβ

ASJC Scopus subject areas

  • Oncology
  • Urology

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