Epigenetic regulation of Myc on retinoic acid receptor beta and PDLIM4 in RWPE1 cells

Meilan He; Donkena Krishna Vanaja; R. Jeffrey Karnes; Charles Y.F. Young

doi:10.1002/pros.21013

Epigenetic regulation of Myc on retinoic acid receptor beta and PDLIM4 in RWPE1 cells

Meilan He, Donkena Krishna Vanaja, R. Jeffrey Karnes, Charles Y.F. Young

Urology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

BACKGROUND. Hypermethylation of CpG islands is a common epigenetic alteration associated with cancer. Tumor suppressor genes retinoic acid receptor beta (RARβ) and PDLIM4 are hypermethylated and silenced in prostate cancer (PCa) tissues and PCa cell lines compared to normal prostate cells. METHODS. In this study, a benign prostate epithelial cell line RWPE1 was used as a model to study the epigenetic regulation of Myc on the RARβ and PDLIM4 promoters. Forced Myc overexpression inhibited the RARβ and PDLIM4 expression. RESULTS. Pyrosequencing study showed that Myc overexpression increased methylation in several CpG sites of both promoters. A DNA methylation inhibitor 5-aza-20-deoxycytidine reversed the epigenetic alteration effect of Myc on both RARβ and PDLIM4. CONCLUSION. The epigenetic regulation of Myc may be related to its up-regulation of the DNA methyltransferase DNMT3a and DNMT3b.

Original language	English (US)
Pages (from-to)	1643-1650
Number of pages	8
Journal	Prostate
Volume	69
Issue number	15
DOIs	https://doi.org/10.1002/pros.21013
State	Published - Nov 1 2009

Keywords

Epigenetic
Myc
PDLIM4
Prostate cancer
RARβ

ASJC Scopus subject areas

Oncology
Urology

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10.1002/pros.21013

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title = "Epigenetic regulation of Myc on retinoic acid receptor beta and PDLIM4 in RWPE1 cells",

abstract = "BACKGROUND. Hypermethylation of CpG islands is a common epigenetic alteration associated with cancer. Tumor suppressor genes retinoic acid receptor beta (RARβ) and PDLIM4 are hypermethylated and silenced in prostate cancer (PCa) tissues and PCa cell lines compared to normal prostate cells. METHODS. In this study, a benign prostate epithelial cell line RWPE1 was used as a model to study the epigenetic regulation of Myc on the RARβ and PDLIM4 promoters. Forced Myc overexpression inhibited the RARβ and PDLIM4 expression. RESULTS. Pyrosequencing study showed that Myc overexpression increased methylation in several CpG sites of both promoters. A DNA methylation inhibitor 5-aza-20-deoxycytidine reversed the epigenetic alteration effect of Myc on both RARβ and PDLIM4. CONCLUSION. The epigenetic regulation of Myc may be related to its up-regulation of the DNA methyltransferase DNMT3a and DNMT3b.",

keywords = "Epigenetic, Myc, PDLIM4, Prostate cancer, RARβ",

author = "Meilan He and Vanaja, {Donkena Krishna} and Karnes, {R. Jeffrey} and Young, {Charles Y.F.}",

year = "2009",

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volume = "69",

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journal = "Prostate",

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T1 - Epigenetic regulation of Myc on retinoic acid receptor beta and PDLIM4 in RWPE1 cells

AU - He, Meilan

AU - Vanaja, Donkena Krishna

AU - Karnes, R. Jeffrey

AU - Young, Charles Y.F.

PY - 2009/11/1

Y1 - 2009/11/1

N2 - BACKGROUND. Hypermethylation of CpG islands is a common epigenetic alteration associated with cancer. Tumor suppressor genes retinoic acid receptor beta (RARβ) and PDLIM4 are hypermethylated and silenced in prostate cancer (PCa) tissues and PCa cell lines compared to normal prostate cells. METHODS. In this study, a benign prostate epithelial cell line RWPE1 was used as a model to study the epigenetic regulation of Myc on the RARβ and PDLIM4 promoters. Forced Myc overexpression inhibited the RARβ and PDLIM4 expression. RESULTS. Pyrosequencing study showed that Myc overexpression increased methylation in several CpG sites of both promoters. A DNA methylation inhibitor 5-aza-20-deoxycytidine reversed the epigenetic alteration effect of Myc on both RARβ and PDLIM4. CONCLUSION. The epigenetic regulation of Myc may be related to its up-regulation of the DNA methyltransferase DNMT3a and DNMT3b.

AB - BACKGROUND. Hypermethylation of CpG islands is a common epigenetic alteration associated with cancer. Tumor suppressor genes retinoic acid receptor beta (RARβ) and PDLIM4 are hypermethylated and silenced in prostate cancer (PCa) tissues and PCa cell lines compared to normal prostate cells. METHODS. In this study, a benign prostate epithelial cell line RWPE1 was used as a model to study the epigenetic regulation of Myc on the RARβ and PDLIM4 promoters. Forced Myc overexpression inhibited the RARβ and PDLIM4 expression. RESULTS. Pyrosequencing study showed that Myc overexpression increased methylation in several CpG sites of both promoters. A DNA methylation inhibitor 5-aza-20-deoxycytidine reversed the epigenetic alteration effect of Myc on both RARβ and PDLIM4. CONCLUSION. The epigenetic regulation of Myc may be related to its up-regulation of the DNA methyltransferase DNMT3a and DNMT3b.

KW - Epigenetic

KW - Myc

KW - PDLIM4

KW - Prostate cancer

KW - RARβ

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DO - 10.1002/pros.21013

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JO - Prostate

JF - Prostate

IS - 15

ER -

Epigenetic regulation of Myc on retinoic acid receptor beta and PDLIM4 in RWPE1 cells

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