Epidermal growth factor (EGF) and somatomedin C regulate G1 progression in competent BALB/c-3T3 cells

Edward B. Leof, Walker Wharton, Judson J. Van Wyk, W. J. Pledger

Research output: Contribution to journalArticle

231 Scopus citations

Abstract

The activity in platelet-poor plasma that allowed density-arrested BALB/c-3T3 cells rendered competent by a transient exposure to platelet-derived growth factor (PDGF) to traverse G1 and enter the S phase has been termed progression activity. Epidermal growth factor (EGF) and somatomedin C-supplemented medium was shown to be capable of replacing the progression activity of 5% platelet-poor plasma (PPP) for competent density-inhibited BALB/c-3T3 cells. Exposure of competent cells to medium supplemented with EGF and somatomedin C reduced the 12 h minimum G1 lag time found in plasma-supplemented medium by 2 h. It is suggested that the reduction in the minimum time required for progression through G1 is due to the availability of free, unbound somatomedin C. Complete G1 traverse required both EGF and somatomedin C; however, the traverse of the last 6 h of G1 and entry into the S phase required only somatomedin C. Though EGF and somatomedin C could replace the G1 phase progression activity of plasma, medium supplemented with EGF and somatomedin C did not support complete cell cycle traverse or growth of sparse cultures of BALB/c-3T3 cells.

Original languageEnglish (US)
Pages (from-to)107-115
Number of pages9
JournalExperimental Cell Research
Volume141
Issue number1
DOIs
StatePublished - Sep 1982

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Epidermal growth factor (EGF) and somatomedin C regulate G1 progression in competent BALB/c-3T3 cells'. Together they form a unique fingerprint.

  • Cite this