Eosinophils promote antiviral immunity in mice infected with influenza A virus

Amali E. Samarasinghe, Rossana C.N. Melo, Susu Duan, Kim S. Le Messurier, Swantje Liedmann, Sherri L. Surman, James J. Lee, Julia L. Hurwitz, Paul G. Thomas, Jonathan A. McCullers

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Eosinophils are multifunctional cells of the innate immune system linked to allergic inflammation. Asthmatics were more likely to be hospitalized but less likely to suffer severe morbidity and mortality during the 2009 influenza pandemic. These epidemiologic findings were recapitulated in a mouse model of fungal asthma wherein infection during heightened allergic inflammation was protective against influenza A virus (IAV) infection and disease. Our goal was to delineate a mechanism(s) by which allergic asthma may alleviate influenza disease outcome, focused on the hypothesis that pulmonary eosinophilia linked with allergic respiratory disease is able to promote antiviral host defenses against the influenza virus. The transfer of eosinophils from the lungs of allergen-sensitized and challenged mice into influenza virus-infected mice resulted in reduced morbidity and viral burden, improved lung compliance, and increased CD8+ T cell numbers in the airways. In vitro assays with primary or bone marrow-derived eosinophils were used to determine eosinophil responses to the virus using the laboratory strain (A/PR/08/1934) or the pandemic strain (A/CA/04/2009) of IAV. Eosinophils were susceptible to IAV infection and responded by activation, piecemeal degranulation, and upregulation of Ag presentation markers. Virus- or viral peptide-exposed eosinophils induced CD8+ T cell proliferation, activation, and effector functions. Our data suggest that eosinophils promote host cellular immunity to reduce influenza virus replication in lungs, thereby providing a novel mechanism by which hosts with allergic asthma may be protected from influenza morbidity.

Original languageEnglish (US)
Pages (from-to)3214-3226
Number of pages13
JournalJournal of Immunology
Volume198
Issue number8
DOIs
StatePublished - Apr 15 2017

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Influenza A virus
Eosinophils
Antiviral Agents
Immunity
Virus Diseases
Orthomyxoviridae
Human Influenza
Asthma
Pandemics
Morbidity
Pulmonary Eosinophilia
Viruses
Inflammation
T-Lymphocytes
Lung Compliance
Lung
Virus Replication
Viral Load
Cellular Immunity
Allergens

ASJC Scopus subject areas

  • Immunology

Cite this

Samarasinghe, A. E., Melo, R. C. N., Duan, S., Le Messurier, K. S., Liedmann, S., Surman, S. L., ... McCullers, J. A. (2017). Eosinophils promote antiviral immunity in mice infected with influenza A virus. Journal of Immunology, 198(8), 3214-3226. https://doi.org/10.4049/jimmunol.1600787

Eosinophils promote antiviral immunity in mice infected with influenza A virus. / Samarasinghe, Amali E.; Melo, Rossana C.N.; Duan, Susu; Le Messurier, Kim S.; Liedmann, Swantje; Surman, Sherri L.; Lee, James J.; Hurwitz, Julia L.; Thomas, Paul G.; McCullers, Jonathan A.

In: Journal of Immunology, Vol. 198, No. 8, 15.04.2017, p. 3214-3226.

Research output: Contribution to journalArticle

Samarasinghe, AE, Melo, RCN, Duan, S, Le Messurier, KS, Liedmann, S, Surman, SL, Lee, JJ, Hurwitz, JL, Thomas, PG & McCullers, JA 2017, 'Eosinophils promote antiviral immunity in mice infected with influenza A virus', Journal of Immunology, vol. 198, no. 8, pp. 3214-3226. https://doi.org/10.4049/jimmunol.1600787
Samarasinghe AE, Melo RCN, Duan S, Le Messurier KS, Liedmann S, Surman SL et al. Eosinophils promote antiviral immunity in mice infected with influenza A virus. Journal of Immunology. 2017 Apr 15;198(8):3214-3226. https://doi.org/10.4049/jimmunol.1600787
Samarasinghe, Amali E. ; Melo, Rossana C.N. ; Duan, Susu ; Le Messurier, Kim S. ; Liedmann, Swantje ; Surman, Sherri L. ; Lee, James J. ; Hurwitz, Julia L. ; Thomas, Paul G. ; McCullers, Jonathan A. / Eosinophils promote antiviral immunity in mice infected with influenza A virus. In: Journal of Immunology. 2017 ; Vol. 198, No. 8. pp. 3214-3226.
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