Eosinophil Shape Change and Secretion

Sergei I. Ochkur, Elizabeth A. Jacobsen, Paige Lacy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The analysis of eosinophil shape change and mediator secretion is a useful tool in understanding how eosinophils respond to immunological stimuli and chemotactic factors. Eosinophils undergo dramatic shape changes, along with secretion of the granule-derived enzyme eosinophil peroxidase (EPX) in response to chemotactic stimuli including platelet-activating factor (PAF) and CCL11 (eotaxin-1). Here, we describe the analysis of eosinophil shape change by confocal microscopy analysis and provide an experimental approach for comparing unstimulated cells with those that have been stimulated to undergo chemotaxis. In addition, we illustrate two different degranulation assays for EPX using OPD and an ELISA technique and show how eosinophil degranulation may be assessed from in vitro as well as ex vivo stimulation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages199-219
Number of pages21
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2241
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Chemokine
  • Chemotaxis
  • Confocal microscopy
  • Degranulation
  • Eosinophil peroxidase
  • Eotaxin
  • Platelet-activating factor
  • Secretion

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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