Eosinophil shape change and secretion

Lian Willetts, Sergei I. Ochkur, Elizabeth A. Jacobsen, James J. Lee, Paige Lacy

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

The analysis of eosinophil shape change and mediator secretion is a useful tool in understanding how eosinophils respond to immunological stimuli and chemotactic factors. Eosinophils undergo dramatic shape changes, along with secretion of the granule-derived enzyme eosinophil peroxidase (EPX) in response to chemotactic stimuli including platelet-activating factor and CCL11 (eotaxin-1). Here, we describe the analysis of eosinophil shape change by confocal microscopy analysis and provide an experimental approach for comparing unstimulated cells with those that have been stimulated to undergo chemotaxis. In addition, we illustrate two different degranulation assays for EPX using OPD and an enzyme-linked immunosorbent assay technique and show how eosinophil degranulation may be assessed from in vitro as well as ex vivo stimulation.

Original languageEnglish (US)
Title of host publicationEosinophils
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages111-128
Number of pages18
ISBN (Print)9781493910151
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1178
ISSN (Print)1064-3745

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Keywords

  • Chemokine
  • Chemotaxis
  • Confocal microscopy
  • Degranulation
  • Eosinophil peroxidase
  • Eotaxin
  • Platelet-activating factor
  • Secretion

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Willetts, L., Ochkur, S. I., Jacobsen, E. A., Lee, J. J., & Lacy, P. (2014). Eosinophil shape change and secretion. In Eosinophils: Methods and Protocols (pp. 111-128). (Methods in Molecular Biology; Vol. 1178). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-1016-8_11