Eosinophil shape change and secretion

Lian Willetts, Sergei I. Ochkur, Elizabeth Jacobsen, James J. Lee, Paige Lacy

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

The analysis of eosinophil shape change and mediator secretion is a useful tool in understanding how eosinophils respond to immunological stimuli and chemotactic factors. Eosinophils undergo dramatic shape changes, along with secretion of the granule-derived enzyme eosinophil peroxidase (EPX) in response to chemotactic stimuli including platelet-activating factor and CCL11 (eotaxin-1). Here, we describe the analysis of eosinophil shape change by confocal microscopy analysis and provide an experimental approach for comparing unstimulated cells with those that have been stimulated to undergo chemotaxis. In addition, we illustrate two different degranulation assays for EPX using OPD and an enzyme-linked immunosorbent assay technique and show how eosinophil degranulation may be assessed from in vitro as well as ex vivo stimulation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages111-128
Number of pages18
Volume1178
ISBN (Print)9781493910151
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1178
ISSN (Print)10643745

Fingerprint

Eosinophils
Eosinophil Peroxidase
Chemokine CCL11
Immunosorbent Techniques
Platelet Activating Factor
Chemotactic Factors
Immunologic Factors
Chemotaxis
Confocal Microscopy
Enzyme-Linked Immunosorbent Assay
Enzymes

Keywords

  • Chemokine
  • Chemotaxis
  • Confocal microscopy
  • Degranulation
  • Eosinophil peroxidase
  • Eotaxin
  • Platelet-activating factor
  • Secretion

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Willetts, L., Ochkur, S. I., Jacobsen, E., Lee, J. J., & Lacy, P. (2014). Eosinophil shape change and secretion. In Methods in Molecular Biology (Vol. 1178, pp. 111-128). (Methods in Molecular Biology; Vol. 1178). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-1016-8_11

Eosinophil shape change and secretion. / Willetts, Lian; Ochkur, Sergei I.; Jacobsen, Elizabeth; Lee, James J.; Lacy, Paige.

Methods in Molecular Biology. Vol. 1178 Humana Press Inc., 2014. p. 111-128 (Methods in Molecular Biology; Vol. 1178).

Research output: Chapter in Book/Report/Conference proceedingChapter

Willetts, L, Ochkur, SI, Jacobsen, E, Lee, JJ & Lacy, P 2014, Eosinophil shape change and secretion. in Methods in Molecular Biology. vol. 1178, Methods in Molecular Biology, vol. 1178, Humana Press Inc., pp. 111-128. https://doi.org/10.1007/978-1-4939-1016-8_11
Willetts L, Ochkur SI, Jacobsen E, Lee JJ, Lacy P. Eosinophil shape change and secretion. In Methods in Molecular Biology. Vol. 1178. Humana Press Inc. 2014. p. 111-128. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-1016-8_11
Willetts, Lian ; Ochkur, Sergei I. ; Jacobsen, Elizabeth ; Lee, James J. ; Lacy, Paige. / Eosinophil shape change and secretion. Methods in Molecular Biology. Vol. 1178 Humana Press Inc., 2014. pp. 111-128 (Methods in Molecular Biology).
@inbook{d8e7ab4bef03451597b82406a8fe6cb7,
title = "Eosinophil shape change and secretion",
abstract = "The analysis of eosinophil shape change and mediator secretion is a useful tool in understanding how eosinophils respond to immunological stimuli and chemotactic factors. Eosinophils undergo dramatic shape changes, along with secretion of the granule-derived enzyme eosinophil peroxidase (EPX) in response to chemotactic stimuli including platelet-activating factor and CCL11 (eotaxin-1). Here, we describe the analysis of eosinophil shape change by confocal microscopy analysis and provide an experimental approach for comparing unstimulated cells with those that have been stimulated to undergo chemotaxis. In addition, we illustrate two different degranulation assays for EPX using OPD and an enzyme-linked immunosorbent assay technique and show how eosinophil degranulation may be assessed from in vitro as well as ex vivo stimulation.",
keywords = "Chemokine, Chemotaxis, Confocal microscopy, Degranulation, Eosinophil peroxidase, Eotaxin, Platelet-activating factor, Secretion",
author = "Lian Willetts and Ochkur, {Sergei I.} and Elizabeth Jacobsen and Lee, {James J.} and Paige Lacy",
year = "2014",
doi = "10.1007/978-1-4939-1016-8_11",
language = "English (US)",
isbn = "9781493910151",
volume = "1178",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "111--128",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Eosinophil shape change and secretion

AU - Willetts, Lian

AU - Ochkur, Sergei I.

AU - Jacobsen, Elizabeth

AU - Lee, James J.

AU - Lacy, Paige

PY - 2014

Y1 - 2014

N2 - The analysis of eosinophil shape change and mediator secretion is a useful tool in understanding how eosinophils respond to immunological stimuli and chemotactic factors. Eosinophils undergo dramatic shape changes, along with secretion of the granule-derived enzyme eosinophil peroxidase (EPX) in response to chemotactic stimuli including platelet-activating factor and CCL11 (eotaxin-1). Here, we describe the analysis of eosinophil shape change by confocal microscopy analysis and provide an experimental approach for comparing unstimulated cells with those that have been stimulated to undergo chemotaxis. In addition, we illustrate two different degranulation assays for EPX using OPD and an enzyme-linked immunosorbent assay technique and show how eosinophil degranulation may be assessed from in vitro as well as ex vivo stimulation.

AB - The analysis of eosinophil shape change and mediator secretion is a useful tool in understanding how eosinophils respond to immunological stimuli and chemotactic factors. Eosinophils undergo dramatic shape changes, along with secretion of the granule-derived enzyme eosinophil peroxidase (EPX) in response to chemotactic stimuli including platelet-activating factor and CCL11 (eotaxin-1). Here, we describe the analysis of eosinophil shape change by confocal microscopy analysis and provide an experimental approach for comparing unstimulated cells with those that have been stimulated to undergo chemotaxis. In addition, we illustrate two different degranulation assays for EPX using OPD and an enzyme-linked immunosorbent assay technique and show how eosinophil degranulation may be assessed from in vitro as well as ex vivo stimulation.

KW - Chemokine

KW - Chemotaxis

KW - Confocal microscopy

KW - Degranulation

KW - Eosinophil peroxidase

KW - Eotaxin

KW - Platelet-activating factor

KW - Secretion

UR - http://www.scopus.com/inward/record.url?scp=84924487076&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924487076&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-1016-8_11

DO - 10.1007/978-1-4939-1016-8_11

M3 - Chapter

SN - 9781493910151

VL - 1178

T3 - Methods in Molecular Biology

SP - 111

EP - 128

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -