Eosinophil ribonucleases and their cutaneous lesion-forming activity

Douglas A. Plager, Mark D P Davis, Amy Andrews, Michael J. Coenen, Terry J. George, Gerald J. Gleich, Kristin M. Leiferman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Eosinophil granule proteins are deposited in cutaneous lesions in many human diseases, but how these proteins contribute to pathophysiology is obscure. We injected eosinophil cationic protein (ECP or RNase 3), eosinophil-derived neurotoxin (EDN or RNase 2), eosinophil peroxidase (EPO), and major basic protein-1 (MBP1) intradermally into guinea pig and rabbit skin. ECP and EDN each induced distinct skin lesions at ≥2.5 μM that began at 2 days, peaking at ∼7 days and persisting up to 6 wk. These lesions were ulcerated (ECP) or crusted (EDN) with marked cellular infiltration. EPO and MBP1 (10 μM) each produced perceptible induration and erythema with moderate cellular infiltration resolving within 2 wk. ECP and EDN localized to dermal cells within 2 days, whereas EPO and MBP1 remained extracellular. Overall, cellular localization and RNase activity of ECP and EDN were critical for lesion formation; differential glycosylation, net cationic charge, or RNase activity alone did not account for lesion formation. Ulcerated lesions from patients with the hypereosinophilic syndrome showed ECP and EDN deposition comparable to that in guinea pig skin. In conclusion, ECP and EDN disrupt skin integrity and cause inflammation. Their presence in ulcerative skin lesions may explain certain findings in human eosinophil-associated diseases.

Original languageEnglish (US)
Pages (from-to)4013-4020
Number of pages8
JournalJournal of Immunology
Volume183
Issue number6
DOIs
StatePublished - Sep 15 2009

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Ribonucleases
Eosinophils
Eosinophil Major Basic Protein
Eosinophil Peroxidase
Skin
Eosinophil Cationic Protein
Eosinophil Granule Proteins
Guinea Pigs
Eosinophil-Derived Neurotoxin
Hypereosinophilic Syndrome
Erythema
Glycosylation
Rabbits
Inflammation
Proteins

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Plager, D. A., Davis, M. D. P., Andrews, A., Coenen, M. J., George, T. J., Gleich, G. J., & Leiferman, K. M. (2009). Eosinophil ribonucleases and their cutaneous lesion-forming activity. Journal of Immunology, 183(6), 4013-4020. https://doi.org/10.4049/jimmunol.0900055

Eosinophil ribonucleases and their cutaneous lesion-forming activity. / Plager, Douglas A.; Davis, Mark D P; Andrews, Amy; Coenen, Michael J.; George, Terry J.; Gleich, Gerald J.; Leiferman, Kristin M.

In: Journal of Immunology, Vol. 183, No. 6, 15.09.2009, p. 4013-4020.

Research output: Contribution to journalArticle

Plager, DA, Davis, MDP, Andrews, A, Coenen, MJ, George, TJ, Gleich, GJ & Leiferman, KM 2009, 'Eosinophil ribonucleases and their cutaneous lesion-forming activity', Journal of Immunology, vol. 183, no. 6, pp. 4013-4020. https://doi.org/10.4049/jimmunol.0900055
Plager DA, Davis MDP, Andrews A, Coenen MJ, George TJ, Gleich GJ et al. Eosinophil ribonucleases and their cutaneous lesion-forming activity. Journal of Immunology. 2009 Sep 15;183(6):4013-4020. https://doi.org/10.4049/jimmunol.0900055
Plager, Douglas A. ; Davis, Mark D P ; Andrews, Amy ; Coenen, Michael J. ; George, Terry J. ; Gleich, Gerald J. ; Leiferman, Kristin M. / Eosinophil ribonucleases and their cutaneous lesion-forming activity. In: Journal of Immunology. 2009 ; Vol. 183, No. 6. pp. 4013-4020.
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