Eosinophil recruitment is associated with IL-5, but not with RANTES, twenty-four hours after allergen challenge

Sanjiv Sur, Hirohito Kita, Gerald J. Gleich, Thomas C. Chenier, Loren W. Hunt

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Abstract

Several lines of evidence suggest that the chemokine RANTES may play a role in eosinophilia observed during allergic inflammation. To test this hypothesis, six patients with allergic asthma were studied. After performing bronchoalveolar lavage in a lung segment (baseline), segmental bronchoprovocation was performed with saline solution in another segment and with ragweed in a third segment. Bronchoalveolar lavage was performed 24 hours later in the saline-challenged (sham) and ragweed-challenged lung segments. The bronchoalveolar lavage fluids from the baseline, sham, and ragweed segments were analyzed for cell counts and for the levels of IL-5, RANTES, and eosinophil-derived neurotoxin IL-5 levels were elevated in the ragweed (984 ± 588 pg/ml) compared with sham segments (2.8 ± 0.2 pg/ml, p = 0.02). Likewise, RANTES levels were elevated in the ragweed (12.93 ± 3.4 pg/ml) compared with the sham segments (3.05 ± 1.19 pg/ml, p = 0.006). The IL-5 levels correlated with both eosinophil numbers (r = 0.90, p < 0.02) and eosinophil-derived neurotoxin levels (r = 0.89) p < 0.02). In contrast, RANTES levels did not correlate with either eosinophil numbers or eosinophil- derived neurotoxin levels. These results indicate that although both IL-5 and RANTES are elevated 24 hours after allergen challenge, only IL-5 correlates with eosinophil recruitment and degranulation.

Original languageEnglish (US)
Pages (from-to)1272-1278
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume97
Issue number6
DOIs
StatePublished - Jan 1 1996

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Keywords

  • EDN
  • IL-5
  • RANTES
  • asthma
  • chemokines
  • cytokines
  • eosinophils
  • late phase reaction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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