Eosinophil infiltration and degranulation in normal human tissue

Masahiko Kato, Gail M. Kephart, Nicholas J. Talley, Jill M. Wagner, Michael G. Sarr, Motoki Bonno, Thomas W. Mcgovern, Gerald J. Gleich

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Localization of eosinophil granule major basic protein by immunofluorescence permits recognition of both eosinophil infiltration and degranulation. Over the past decade and a half, our laboratory has shown that eosinophil infiltration and degranulation occur in many diseased tissues in humans; among normal tissues studied as controls, only the gut showed striking eosinophil infiltration and degranulation. Using an indirect immunofluorescence procedure for the detection of major basic protein, we extended our analyses of normal human tissues to include tissues from essentially all body organs; a total of 117 biopsy/autopsy specimens were analyzed. To determine whether the method of tissue procurement affected the level of eosinophil degranulation in the normal gastrointestinal tract, normal proximal jejunum from six patients was biopsied using either an endoscopic forceps or a scalpel at the time of elective surgery and examined by immunofluorescence. Spleen, lymph node, and thymus tissues showed eosinophil infiltration with scant evidence of degranulation, but the only organ showing both eosinophil infiltration and remarkable degranulation was the gastrointestinal tract. Eosinophil degranulation was significantly increased in specimens obtained by endoscopic forceps compared to those obtained by scalpel (P = 0.021). These results indicate that tissue procurement methods affect the degree of eosinophil degranulation in the gut. Thus, among normal human body organs, both eosinophil infiltration and appreciable degranulation consistently occur only in the gut.

Original languageEnglish (US)
Pages (from-to)418-425
Number of pages8
JournalAnatomical Record
Volume252
Issue number3
DOIs
StatePublished - Nov 1998

Fingerprint

eosinophils
Eosinophils
infiltration
scalpels
fluorescent antibody technique
Tissue and Organ Procurement
digestive system
Surgical Instruments
Fluorescent Antibody Technique
Gastrointestinal Tract
gastrointestinal system
Eosinophil Major Basic Protein
protein
tissues
tissue
Jejunum
Indirect Fluorescent Antibody Technique
Human Body
Thymus Gland
jejunum

Keywords

  • Eosinophils
  • Gastrointestinal tract
  • Major basic protein
  • Normal human tissue

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Kato, M., Kephart, G. M., Talley, N. J., Wagner, J. M., Sarr, M. G., Bonno, M., ... Gleich, G. J. (1998). Eosinophil infiltration and degranulation in normal human tissue. Anatomical Record, 252(3), 418-425. https://doi.org/10.1002/(SICI)1097-0185(199811)252:3<418::AID-AR10>3.0.CO;2-1

Eosinophil infiltration and degranulation in normal human tissue. / Kato, Masahiko; Kephart, Gail M.; Talley, Nicholas J.; Wagner, Jill M.; Sarr, Michael G.; Bonno, Motoki; Mcgovern, Thomas W.; Gleich, Gerald J.

In: Anatomical Record, Vol. 252, No. 3, 11.1998, p. 418-425.

Research output: Contribution to journalArticle

Kato, M, Kephart, GM, Talley, NJ, Wagner, JM, Sarr, MG, Bonno, M, Mcgovern, TW & Gleich, GJ 1998, 'Eosinophil infiltration and degranulation in normal human tissue', Anatomical Record, vol. 252, no. 3, pp. 418-425. https://doi.org/10.1002/(SICI)1097-0185(199811)252:3<418::AID-AR10>3.0.CO;2-1
Kato, Masahiko ; Kephart, Gail M. ; Talley, Nicholas J. ; Wagner, Jill M. ; Sarr, Michael G. ; Bonno, Motoki ; Mcgovern, Thomas W. ; Gleich, Gerald J. / Eosinophil infiltration and degranulation in normal human tissue. In: Anatomical Record. 1998 ; Vol. 252, No. 3. pp. 418-425.
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