Eosinophil granule proteins in peripheral blood granulocytes

R. I. Abu-Ghazaleh, S. L. Dunnette, D. A. Loegering, J. L. Checkel, Hirohito Kita, L. L. Thomas, G. J. Gleich

Research output: Contribution to journal › Article

147 Citations (Scopus)

Abstract

Eosinophils contain four principal cationic proteins, major basic protein (MBP), eosinophil-derived neurotoxin (EDN), eosinophil cationic protein (ECP), and eosinophil peroxidase (EPO). To determine the quantities of these proteins in granulocytes and whether they are specific to eosinophils, their concentrations in lysates of human granulocytes were measured using specific radioimmunoassays. The effect of different methods for eosinophil lysis on the recovery of the proteins was also studied. Maximal recovery occurred at pH 2 for MBP and pH 5.6 for the other granule proteins. The proteins cosedimented with eosinophils and their concentrations (X̄ ± SEM) in ng/106 eosinophils (and in nM/106 eosinophils) were: MBP, 8,982 ± 611 (641.6); EDN, 3,283 ± 116 (178.4); ECP, 5,269 ± 283 (250.9); and EPO, 12,174 ± 859 (171.5). Basophils from a normal person contained (in ng/106 cells) MBP, 2,374; EDN, 214; ECP, 77; and EPO, 17. Highly purified neutrophils contained (in ng/106 cells) MBP, 3 ± 0.5; EDN, 72 ± 9; and ECP, 50 ± 12. Therefore we conclude that these proteins are mainly expressed in eosinophils, but that certain ones are present in basophils and neutrophils.

Original languageEnglish (US)
Pages (from-to)611-618
Number of pages8
JournalJournal of Leukocyte Biology
Volume52
Issue number6
StatePublished - 1992

Fingerprint

Eosinophil Granule Proteins
Granulocytes
Eosinophil-Derived Neurotoxin
Eosinophils
Eosinophil Cationic Protein
Eosinophil Peroxidase
Proteins
Basophils
Neutrophils
Eosinophil Major Basic Protein
Radioimmunoassay

Keywords

  • basophils
  • cationic protein
  • eosinophil
  • eosinophil peroxidase
  • eosinophil-derived neurotoxin
  • major basic protein
  • neutrophils

ASJC Scopus subject areas

  • Cell Biology

Cite this

Abu-Ghazaleh, R. I., Dunnette, S. L., Loegering, D. A., Checkel, J. L., Kita, H., Thomas, L. L., & Gleich, G. J. (1992). Eosinophil granule proteins in peripheral blood granulocytes. Journal of Leukocyte Biology, 52(6), 611-618.

Eosinophil granule proteins in peripheral blood granulocytes. / Abu-Ghazaleh, R. I.; Dunnette, S. L.; Loegering, D. A.; Checkel, J. L.; Kita, Hirohito; Thomas, L. L.; Gleich, G. J.

In: Journal of Leukocyte Biology, Vol. 52, No. 6, 1992, p. 611-618.

Research output: Contribution to journal › Article

Abu-Ghazaleh, RI, Dunnette, SL, Loegering, DA, Checkel, JL, Kita, H, Thomas, LL & Gleich, GJ 1992, 'Eosinophil granule proteins in peripheral blood granulocytes', Journal of Leukocyte Biology, vol. 52, no. 6, pp. 611-618.
Abu-Ghazaleh RI, Dunnette SL, Loegering DA, Checkel JL, Kita H, Thomas LL et al. Eosinophil granule proteins in peripheral blood granulocytes. Journal of Leukocyte Biology. 1992;52(6):611-618.
Abu-Ghazaleh, R. I. ; Dunnette, S. L. ; Loegering, D. A. ; Checkel, J. L. ; Kita, Hirohito ; Thomas, L. L. ; Gleich, G. J. / Eosinophil granule proteins in peripheral blood granulocytes. In: Journal of Leukocyte Biology. 1992 ; Vol. 52, No. 6. pp. 611-618.
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AU - Loegering, D. A.

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AU - Kita, Hirohito

AU - Thomas, L. L.

AU - Gleich, G. J.

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N2 - Eosinophils contain four principal cationic proteins, major basic protein (MBP), eosinophil-derived neurotoxin (EDN), eosinophil cationic protein (ECP), and eosinophil peroxidase (EPO). To determine the quantities of these proteins in granulocytes and whether they are specific to eosinophils, their concentrations in lysates of human granulocytes were measured using specific radioimmunoassays. The effect of different methods for eosinophil lysis on the recovery of the proteins was also studied. Maximal recovery occurred at pH 2 for MBP and pH 5.6 for the other granule proteins. The proteins cosedimented with eosinophils and their concentrations (X̄ ± SEM) in ng/106 eosinophils (and in nM/106 eosinophils) were: MBP, 8,982 ± 611 (641.6); EDN, 3,283 ± 116 (178.4); ECP, 5,269 ± 283 (250.9); and EPO, 12,174 ± 859 (171.5). Basophils from a normal person contained (in ng/106 cells) MBP, 2,374; EDN, 214; ECP, 77; and EPO, 17. Highly purified neutrophils contained (in ng/106 cells) MBP, 3 ± 0.5; EDN, 72 ± 9; and ECP, 50 ± 12. Therefore we conclude that these proteins are mainly expressed in eosinophils, but that certain ones are present in basophils and neutrophils.

AB - Eosinophils contain four principal cationic proteins, major basic protein (MBP), eosinophil-derived neurotoxin (EDN), eosinophil cationic protein (ECP), and eosinophil peroxidase (EPO). To determine the quantities of these proteins in granulocytes and whether they are specific to eosinophils, their concentrations in lysates of human granulocytes were measured using specific radioimmunoassays. The effect of different methods for eosinophil lysis on the recovery of the proteins was also studied. Maximal recovery occurred at pH 2 for MBP and pH 5.6 for the other granule proteins. The proteins cosedimented with eosinophils and their concentrations (X̄ ± SEM) in ng/106 eosinophils (and in nM/106 eosinophils) were: MBP, 8,982 ± 611 (641.6); EDN, 3,283 ± 116 (178.4); ECP, 5,269 ± 283 (250.9); and EPO, 12,174 ± 859 (171.5). Basophils from a normal person contained (in ng/106 cells) MBP, 2,374; EDN, 214; ECP, 77; and EPO, 17. Highly purified neutrophils contained (in ng/106 cells) MBP, 3 ± 0.5; EDN, 72 ± 9; and ECP, 50 ± 12. Therefore we conclude that these proteins are mainly expressed in eosinophils, but that certain ones are present in basophils and neutrophils.

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