Eosinophil deficiency compromises parasite survival in chronic nematode infection

Valeria Fabre, Daniel P. Beiting, Susan K. Bliss, Nebiat G. Gebreselassie, Lucille F. Gagliardo, Nancy A Lee, James J. Lee, Judith A. Appleton

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Immune responses elicited by parasitic worms share many features with those of chronic allergy. Eosinophils contribute to the inflammation that occurs in both types of disease, and helminths can be damaged or killed by toxic products released by eosinophils in vitro. Such observations inform the widely held view that eosinophils protect the host against parasitic worms. The mouse is a natural host for Trichinella spiralis, a worm that establishes chronic infection in skeletal muscle. We tested the influence of eosinophils on T. spiralis infection in two mouse strains in which the eosinophil lineage is ablated. Eosinophils were prominent in infiltrates surrounding infected muscle cells of wild-type mice; however, in the absence of eosinophils T. spiralis muscle larvae died in large numbers. Parasite death correlated with enhanced IFN-γ and decreased IL-4 production. Larval survival improved when mice were treated with inhibitors of inducible NO synthase, implicating the NO pathway in parasite clearance. Thus, the long-standing paradigm of eosinophil toxicity in nematode infection requires reevaluation, as our results suggest that eosinophils may influence the immune response in a manner that would sustain chronic infection and insure worm survival in the host population. Such a mechanism may be deployed by other parasitic worms that depend upon chronic infection for survival.

Original languageEnglish (US)
Pages (from-to)1577-1583
Number of pages7
JournalJournal of Immunology
Volume182
Issue number3
StatePublished - Feb 1 2009

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Nematode Infections
Eosinophils
Parasites
Helminths
Trichinella spiralis
Infection
Poisons
Nitric Oxide Synthase
Interleukin-4
Muscle Cells
Larva
Hypersensitivity
Skeletal Muscle
Inflammation
Muscles

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Fabre, V., Beiting, D. P., Bliss, S. K., Gebreselassie, N. G., Gagliardo, L. F., Lee, N. A., ... Appleton, J. A. (2009). Eosinophil deficiency compromises parasite survival in chronic nematode infection. Journal of Immunology, 182(3), 1577-1583.

Eosinophil deficiency compromises parasite survival in chronic nematode infection. / Fabre, Valeria; Beiting, Daniel P.; Bliss, Susan K.; Gebreselassie, Nebiat G.; Gagliardo, Lucille F.; Lee, Nancy A; Lee, James J.; Appleton, Judith A.

In: Journal of Immunology, Vol. 182, No. 3, 01.02.2009, p. 1577-1583.

Research output: Contribution to journalArticle

Fabre, V, Beiting, DP, Bliss, SK, Gebreselassie, NG, Gagliardo, LF, Lee, NA, Lee, JJ & Appleton, JA 2009, 'Eosinophil deficiency compromises parasite survival in chronic nematode infection', Journal of Immunology, vol. 182, no. 3, pp. 1577-1583.
Fabre V, Beiting DP, Bliss SK, Gebreselassie NG, Gagliardo LF, Lee NA et al. Eosinophil deficiency compromises parasite survival in chronic nematode infection. Journal of Immunology. 2009 Feb 1;182(3):1577-1583.
Fabre, Valeria ; Beiting, Daniel P. ; Bliss, Susan K. ; Gebreselassie, Nebiat G. ; Gagliardo, Lucille F. ; Lee, Nancy A ; Lee, James J. ; Appleton, Judith A. / Eosinophil deficiency compromises parasite survival in chronic nematode infection. In: Journal of Immunology. 2009 ; Vol. 182, No. 3. pp. 1577-1583.
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