Enzymatic determination of total 3αhydroxy bile acids in faeces: Validation in healthy subjects of a rapid method suitable for clinical routine purpose

A. Malchow-Møller, S. Arffmann, Nicholas F La Russo, E. Krag

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A method for determining faecal bile acids, suitable for clinical purposes, is introduced. The analysis uses a 0.2-g stool specimen, a simple extraction procedure, and 3αsteroid dehydrogenase determination. The method, which is rapid, has been validated by gas-liquid chromatography and by recovery of internal standards. Stool examination was done in 16 healthy volunteers on free diet and in 25 patients with non-gastrointestinal diseases who were on a fat- and fibre-fixed diet. No difference was found between the two groups, so the data were pooled, and the normal reference interval (X ± 2S.D.) for faecal bile acid output was calculated to be 0-975 μmol/24h.

Original languageEnglish (US)
Pages (from-to)331-333
Number of pages3
JournalScandinavian Journal of Gastroenterology
Volume17
Issue number3
DOIs
StatePublished - 1982

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Bile Acids and Salts
Feces
Healthy Volunteers
Diet
Gas Chromatography
Oxidoreductases
Fats

Keywords

  • Bile acids
  • Faeces

ASJC Scopus subject areas

  • Gastroenterology

Cite this

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abstract = "A method for determining faecal bile acids, suitable for clinical purposes, is introduced. The analysis uses a 0.2-g stool specimen, a simple extraction procedure, and 3αsteroid dehydrogenase determination. The method, which is rapid, has been validated by gas-liquid chromatography and by recovery of internal standards. Stool examination was done in 16 healthy volunteers on free diet and in 25 patients with non-gastrointestinal diseases who were on a fat- and fibre-fixed diet. No difference was found between the two groups, so the data were pooled, and the normal reference interval (X ± 2S.D.) for faecal bile acid output was calculated to be 0-975 μmol/24h.",
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T2 - Validation in healthy subjects of a rapid method suitable for clinical routine purpose

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AU - Arffmann, S.

AU - La Russo, Nicholas F

AU - Krag, E.

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AB - A method for determining faecal bile acids, suitable for clinical purposes, is introduced. The analysis uses a 0.2-g stool specimen, a simple extraction procedure, and 3αsteroid dehydrogenase determination. The method, which is rapid, has been validated by gas-liquid chromatography and by recovery of internal standards. Stool examination was done in 16 healthy volunteers on free diet and in 25 patients with non-gastrointestinal diseases who were on a fat- and fibre-fixed diet. No difference was found between the two groups, so the data were pooled, and the normal reference interval (X ± 2S.D.) for faecal bile acid output was calculated to be 0-975 μmol/24h.

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