Enhancement of ultrasonically induced cell transfection with artificial cavitation nuclei

William J. Greenleaf, Mark E. Bolander, Gobinda Sarkar, Mary B. Goldring, James F. Greenleaf

Research output: Contribution to journalConference articlepeer-review

Abstract

Ultrasound mediated gene transfection in cultured immortalized human chondrocytes was enhanced three-fold using artificial cavitation nuclei in the form of Albunex. 1.0 MHz ultrasound transmitted through the bottoms of six-well culture plates containing media with green fluorescent protein reporter gene plasmids at a concentration of 40 pg/ml and Albunex at 50×106 bubbles/ml, exhibited a peak transfection efficiency of about 50% of the living cells when exposed to 4×105 Pascals spatial average peak pressure (SAPP) for 20 minutes. Using these parameters, transfection efficiency increased linearly with ultrasound exposure pressure with a transfection threshold observed at a SAPP of 105 Pascals. Adding fresh Albunex at 50×106 bubbles/ml prior to sequential one second 3.2 or 4.0×105 Pascal exposures increased transfection efficiency by 15% for each of three consecutive exposures. Efficient in vitro and perhaps in vivo transfection appears possible with ultrasound.

Original languageEnglish (US)
Pages (from-to)1365-1369
Number of pages5
JournalProceedings of the IEEE Ultrasonics Symposium
Volume2
StatePublished - 1997
EventProceedings of the 1997 IEEE Ultrasonics Symposium. Part 1 (of 2) - Toronto, Can
Duration: Oct 5 1997Oct 8 1997

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

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