Abstract
Ultrasound mediated gene transfection in cultured immortalized human chondrocytes was enhanced three-fold using artificial cavitation nuclei in the form of Albunex. 1.0 MHz ultrasound transmitted through the bottoms of six-well culture plates containing media with green fluorescent protein reporter gene plasmids at a concentration of 40 pg/ml and Albunex at 50×106 bubbles/ml, exhibited a peak transfection efficiency of about 50% of the living cells when exposed to 4×105 Pascals spatial average peak pressure (SAPP) for 20 minutes. Using these parameters, transfection efficiency increased linearly with ultrasound exposure pressure with a transfection threshold observed at a SAPP of 105 Pascals. Adding fresh Albunex at 50×106 bubbles/ml prior to sequential one second 3.2 or 4.0×105 Pascal exposures increased transfection efficiency by 15% for each of three consecutive exposures. Efficient in vitro and perhaps in vivo transfection appears possible with ultrasound.
Original language | English (US) |
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Pages (from-to) | 1365-1369 |
Number of pages | 5 |
Journal | Proceedings of the IEEE Ultrasonics Symposium |
Volume | 2 |
State | Published - 1997 |
Event | Proceedings of the 1997 IEEE Ultrasonics Symposium. Part 1 (of 2) - Toronto, Can Duration: Oct 5 1997 → Oct 8 1997 |
ASJC Scopus subject areas
- Acoustics and Ultrasonics