TY - JOUR
T1 - Enhanced renal cortical vascularization in experimental hypercholesterolemia
AU - Bentley, Michael D.
AU - Rodriguez-Porcel, Martin
AU - Lerman, Amir
AU - Hershman Sarafov, Mirit
AU - Romero, J. Carlos
AU - Pelaez, Laura I.
AU - Grande, Joseph P.
AU - Ritman, Erik L.
AU - Lerman, Lilach O.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2002/3
Y1 - 2002/3
N2 - Background. Experimental hypercholesterolemia is associated with pro-inflammatory changes and impaired regulation of tissue perfusion, which may lead to neovascularization. However, it is yet unknown whether such changes take place in the kidney. In this study, using a novel three-dimensional (3-D) micro computed-tomography technique we tested the hypothesis that hypercholesterolemia was associated with increased microvascular density in the renal cortex. Methods. Kidneys were excised from pigs after 12 weeks of either a normal (N = 6) or high cholesterol (HC; N = 5) diet, histology slides processed, and a segmental renal artery injected with a radio-opaque intravascular silicone polymer. Renal samples were scanned with micro computed-tomography, transverse and three-dimensional images were reconstructed, and microvessels (80 to 360 μm in diameter) counted in situ. Results. Serum cholesterol levels were significantly higher in hypercholesterolemic compared to normal pigs (383 ± 76 vs. 81 ± 7 mg/dL, P < 0.01), and microvascular spatial density was significantly higher in their inner and middle renal cortex (189 ± 7 vs. 126 ± 6 microvessels/cm2, P < 0.0001). Hypercholesterolemic kidneys also showed mild interstitial mononuclear infiltration and heavier immunostaining of vascular endothelial growth factor, but no other signs of morphological damage. Conclusions. These results demonstrate that early diet-induced hypercholesterolemia is associated with increased microvascular density in the renal cortex, which precedes signs of overt renal morphological damage. These alterations may potentially affect regulation and/or spatial distribution of intrarenal blood flow in hypercholesterolemia, and may participate in renal disease progression.
AB - Background. Experimental hypercholesterolemia is associated with pro-inflammatory changes and impaired regulation of tissue perfusion, which may lead to neovascularization. However, it is yet unknown whether such changes take place in the kidney. In this study, using a novel three-dimensional (3-D) micro computed-tomography technique we tested the hypothesis that hypercholesterolemia was associated with increased microvascular density in the renal cortex. Methods. Kidneys were excised from pigs after 12 weeks of either a normal (N = 6) or high cholesterol (HC; N = 5) diet, histology slides processed, and a segmental renal artery injected with a radio-opaque intravascular silicone polymer. Renal samples were scanned with micro computed-tomography, transverse and three-dimensional images were reconstructed, and microvessels (80 to 360 μm in diameter) counted in situ. Results. Serum cholesterol levels were significantly higher in hypercholesterolemic compared to normal pigs (383 ± 76 vs. 81 ± 7 mg/dL, P < 0.01), and microvascular spatial density was significantly higher in their inner and middle renal cortex (189 ± 7 vs. 126 ± 6 microvessels/cm2, P < 0.0001). Hypercholesterolemic kidneys also showed mild interstitial mononuclear infiltration and heavier immunostaining of vascular endothelial growth factor, but no other signs of morphological damage. Conclusions. These results demonstrate that early diet-induced hypercholesterolemia is associated with increased microvascular density in the renal cortex, which precedes signs of overt renal morphological damage. These alterations may potentially affect regulation and/or spatial distribution of intrarenal blood flow in hypercholesterolemia, and may participate in renal disease progression.
KW - Hypercholesterolemia
KW - Kidney
KW - Micro computed tomography
KW - Microcirculation
KW - Neovascularization
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U2 - 10.1046/j.1523-1755.2002.00211.x
DO - 10.1046/j.1523-1755.2002.00211.x
M3 - Article
C2 - 11849461
AN - SCOPUS:0036183670
VL - 61
SP - 1056
EP - 1063
JO - Kidney International
JF - Kidney International
SN - 0085-2538
IS - 3
ER -