Enhanced prostacyclin formation and Wnt signaling in sclerostin deficient osteocytes and bone

Zachary C. Ryan, Theodore A. Craig, Jeffrey L. Salisbury, Lomeli R. Carpio, Meghan McGee-Lawrence, Jennifer J. Westendorf, Rajiv Kumar

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We show that prostacyclin production is increased in bone and osteocytes from sclerostin (Sost) knockout mice which have greatly increased bone mass. The addition of prostacyclin or a prostacyclin analog to bone forming osteoblasts enhances differentiation and matrix mineralization of osteoblasts. The increase in prostacyclin synthesis is linked to increases in β-catenin concentrations and activity as shown by enhanced binding of lymphoid enhancer factor, Lef1, to promoter elements within the prostacyclin synthase promoter. Blockade of Wnt signaling reduces prostacyclin production in osteocytes. Increased prostacyclin production by osteocytes from sclerostin deficient mice could potentially contribute to the increased bone formation seen in this condition.

Original languageEnglish (US)
Pages (from-to)83-88
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume448
Issue number1
DOIs
StatePublished - May 23 2014

Keywords

  • Osteocytes
  • Prostacyclin
  • Sclerostin deficiency
  • Wnt

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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