Enhanced cytotoxicity without internuclear spread of adenovirus upon cell fusion by measles virus glycoproteins

German P. Horn, Sompong Vongpunsawad, Evelyn Kornmann, Barbara Fritz, Dirk P. Dittmer, Roberto Cattaneo, Matthias Dobbelstein

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The efficiency of viruses in cancer therapy is enhanced by proteins that mediate the fusion of infected cells with their neighbors. It was reported that replication-competent adenovirus particles can spread between nuclei within fusion-generated syncytia. To assess this conjecture, we generated fusogenic adenoviruses that express a balanced ratio of the F and H glycoproteins of measles virus. The viruses displayed enhanced cytotoxicity but largely unchanged replication efficiencies compared to a nonfusogenic virus. Most notably, the virus genomes did not spread through fusion-generated multinuclear cells. Hence, adenovirus replication in syncytia remains largely restricted to initially transduced nuclei.

Original languageEnglish (US)
Pages (from-to)1911-1917
Number of pages7
JournalJournal of virology
Volume79
Issue number3
DOIs
StatePublished - Feb 1 2005

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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