Enhanced cell penetration of acid-degradable particles functionalized with cell-penetrating peptides

Jessica L. Cohen, Adah Almutairi, Joel A. Cohen, Matt Bernstein, Steven L. Brody, Daniel P. Schuster, Jean M.J. Fréchet

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Biopharmaceuticals, such as proteins and DNA, have demonstrated their potential to prevent and cure diseases. The success of such therapeutic agents hinges upon their ability to cross complex barriers in the body and reach their target intact. In order to reap the full benefits of these therapeutic agents, a delivery vehicle capable of delivering cargo to all cell types, both phagocytic and non-phagocytic, is needed. This article presents the synthesis and evaluation of a microparticle delivery vehicle capable of cell penetration and sub-cellular triggered release of an encapsulated payload. pH-sensitive polyacrylamide particles functionalized with a polyarginine cell-penetrating peptide (CPP) were synthesized. The incorporation of a CPP into the microparticles led to efficient uptake by non-phagocytic cells in culture. In addition, the CPP-modificd particles showed no cytotoxic effects at concentrations used in this study. The results suggest that these particles may provide a vehicle for the successful delivery of therapeutic agents to various cell types.

Original languageEnglish (US)
Pages (from-to)876-881
Number of pages6
JournalBioconjugate Chemistry
Volume19
Issue number4
DOIs
StatePublished - Apr 1 2008

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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    Cohen, J. L., Almutairi, A., Cohen, J. A., Bernstein, M., Brody, S. L., Schuster, D. P., & Fréchet, J. M. J. (2008). Enhanced cell penetration of acid-degradable particles functionalized with cell-penetrating peptides. Bioconjugate Chemistry, 19(4), 876-881. https://doi.org/10.1021/bc700414j