Enhanced cell penetration of acid-degradable particles functionalized with cell-penetrating peptides

Jessica L. Cohen, Adah Almutairi, Joel A. Cohen, Matthew A Bernstein, Steven L. Brody, Daniel P. Schuster, Jean M.J. Fréchet

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Biopharmaceuticals, such as proteins and DNA, have demonstrated their potential to prevent and cure diseases. The success of such therapeutic agents hinges upon their ability to cross complex barriers in the body and reach their target intact. In order to reap the full benefits of these therapeutic agents, a delivery vehicle capable of delivering cargo to all cell types, both phagocytic and non-phagocytic, is needed. This article presents the synthesis and evaluation of a microparticle delivery vehicle capable of cell penetration and sub-cellular triggered release of an encapsulated payload. pH-sensitive polyacrylamide particles functionalized with a polyarginine cell-penetrating peptide (CPP) were synthesized. The incorporation of a CPP into the microparticles led to efficient uptake by non-phagocytic cells in culture. In addition, the CPP-modificd particles showed no cytotoxic effects at concentrations used in this study. The results suggest that these particles may provide a vehicle for the successful delivery of therapeutic agents to various cell types.

Original languageEnglish (US)
Pages (from-to)876-881
Number of pages6
JournalBioconjugate Chemistry
Volume19
Issue number4
DOIs
StatePublished - Apr 1 2008
Externally publishedYes

Fingerprint

Cell-Penetrating Peptides
Peptides
Acids
Hinges
Phagocytes
Polyacrylates
DNA
Therapeutics
Cell Culture Techniques
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Cohen, J. L., Almutairi, A., Cohen, J. A., Bernstein, M. A., Brody, S. L., Schuster, D. P., & Fréchet, J. M. J. (2008). Enhanced cell penetration of acid-degradable particles functionalized with cell-penetrating peptides. Bioconjugate Chemistry, 19(4), 876-881. https://doi.org/10.1021/bc700414j

Enhanced cell penetration of acid-degradable particles functionalized with cell-penetrating peptides. / Cohen, Jessica L.; Almutairi, Adah; Cohen, Joel A.; Bernstein, Matthew A; Brody, Steven L.; Schuster, Daniel P.; Fréchet, Jean M.J.

In: Bioconjugate Chemistry, Vol. 19, No. 4, 01.04.2008, p. 876-881.

Research output: Contribution to journalArticle

Cohen, JL, Almutairi, A, Cohen, JA, Bernstein, MA, Brody, SL, Schuster, DP & Fréchet, JMJ 2008, 'Enhanced cell penetration of acid-degradable particles functionalized with cell-penetrating peptides', Bioconjugate Chemistry, vol. 19, no. 4, pp. 876-881. https://doi.org/10.1021/bc700414j
Cohen, Jessica L. ; Almutairi, Adah ; Cohen, Joel A. ; Bernstein, Matthew A ; Brody, Steven L. ; Schuster, Daniel P. ; Fréchet, Jean M.J. / Enhanced cell penetration of acid-degradable particles functionalized with cell-penetrating peptides. In: Bioconjugate Chemistry. 2008 ; Vol. 19, No. 4. pp. 876-881.
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