Abstract
Biopharmaceuticals, such as proteins and DNA, have demonstrated their potential to prevent and cure diseases. The success of such therapeutic agents hinges upon their ability to cross complex barriers in the body and reach their target intact. In order to reap the full benefits of these therapeutic agents, a delivery vehicle capable of delivering cargo to all cell types, both phagocytic and non-phagocytic, is needed. This article presents the synthesis and evaluation of a microparticle delivery vehicle capable of cell penetration and sub-cellular triggered release of an encapsulated payload. pH-sensitive polyacrylamide particles functionalized with a polyarginine cell-penetrating peptide (CPP) were synthesized. The incorporation of a CPP into the microparticles led to efficient uptake by non-phagocytic cells in culture. In addition, the CPP-modificd particles showed no cytotoxic effects at concentrations used in this study. The results suggest that these particles may provide a vehicle for the successful delivery of therapeutic agents to various cell types.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 876-881 |
| Number of pages | 6 |
| Journal | Bioconjugate Chemistry |
| Volume | 19 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1 2008 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Pharmacology
- Pharmaceutical Science
- Organic Chemistry
Cite this
Enhanced cell penetration of acid-degradable particles functionalized with cell-penetrating peptides. / Cohen, Jessica L.; Almutairi, Adah; Cohen, Joel A.; Bernstein, Matthew A; Brody, Steven L.; Schuster, Daniel P.; Fréchet, Jean M.J.
In: Bioconjugate Chemistry, Vol. 19, No. 4, 01.04.2008, p. 876-881.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced cell penetration of acid-degradable particles functionalized with cell-penetrating peptides
AU - Cohen, Jessica L.
AU - Almutairi, Adah
AU - Cohen, Joel A.
AU - Bernstein, Matthew A
AU - Brody, Steven L.
AU - Schuster, Daniel P.
AU - Fréchet, Jean M.J.
PY - 2008/4/1
Y1 - 2008/4/1
N2 - Biopharmaceuticals, such as proteins and DNA, have demonstrated their potential to prevent and cure diseases. The success of such therapeutic agents hinges upon their ability to cross complex barriers in the body and reach their target intact. In order to reap the full benefits of these therapeutic agents, a delivery vehicle capable of delivering cargo to all cell types, both phagocytic and non-phagocytic, is needed. This article presents the synthesis and evaluation of a microparticle delivery vehicle capable of cell penetration and sub-cellular triggered release of an encapsulated payload. pH-sensitive polyacrylamide particles functionalized with a polyarginine cell-penetrating peptide (CPP) were synthesized. The incorporation of a CPP into the microparticles led to efficient uptake by non-phagocytic cells in culture. In addition, the CPP-modificd particles showed no cytotoxic effects at concentrations used in this study. The results suggest that these particles may provide a vehicle for the successful delivery of therapeutic agents to various cell types.
AB - Biopharmaceuticals, such as proteins and DNA, have demonstrated their potential to prevent and cure diseases. The success of such therapeutic agents hinges upon their ability to cross complex barriers in the body and reach their target intact. In order to reap the full benefits of these therapeutic agents, a delivery vehicle capable of delivering cargo to all cell types, both phagocytic and non-phagocytic, is needed. This article presents the synthesis and evaluation of a microparticle delivery vehicle capable of cell penetration and sub-cellular triggered release of an encapsulated payload. pH-sensitive polyacrylamide particles functionalized with a polyarginine cell-penetrating peptide (CPP) were synthesized. The incorporation of a CPP into the microparticles led to efficient uptake by non-phagocytic cells in culture. In addition, the CPP-modificd particles showed no cytotoxic effects at concentrations used in this study. The results suggest that these particles may provide a vehicle for the successful delivery of therapeutic agents to various cell types.
UR - http://www.scopus.com/inward/record.url?scp=42949116303&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42949116303&partnerID=8YFLogxK
U2 - 10.1021/bc700414j
DO - 10.1021/bc700414j
M3 - Article
C2 - 18318462
AN - SCOPUS:42949116303
VL - 19
SP - 876
EP - 881
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
SN - 1043-1802
IS - 4
ER -