Enhanced biological cathodoluminescence

Phyllis J. Fisher, William S. Wessels, Allan B Dietz, Franklyn G. Prendergast

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have combined the specificity of antibody labeling, the power of fluorescence detection, and the resolution of scanning electron microscopy (SEM) to identify antigenic sites on nanometer-scale features of mammalian cells. Cathodoluminescence (CL) detection in SEM was used to locate fluorophores bound to antibodies specific for cell surface epitopes. Sample preparation and instrument setup were optimized to yield the maximum luminescence compatible with a high definition secondary electron image. Separable CL component distances of less than 300 nm have been calculated. Antibody-specific fluorophores are associated with unique morphological features on a human dendritic cell. This technology provides a tool to identify the relationship between cell surface structures and receptor-ligand binding or other antigen-defined physiological states.

Original languageEnglish (US)
Pages (from-to)1901-1908
Number of pages8
JournalOptics Communications
Volume281
Issue number7
DOIs
StatePublished - Apr 1 2008

Fingerprint

Cathodoluminescence
cathodoluminescence
antibodies
Antibodies
Fluorophores
cells
Epitopes
Scanning electron microscopy
scanning electron microscopy
antigens
Antigens
Surface structure
Labeling
marking
Luminescence
Fluorescence
Ligands
Cells
luminescence
fluorescence

Keywords

  • Antigen detection
  • Cathodoluminescence
  • Cell morphology
  • Fluorescence
  • Mammalian cells
  • Resolution
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Fisher, P. J., Wessels, W. S., Dietz, A. B., & Prendergast, F. G. (2008). Enhanced biological cathodoluminescence. Optics Communications, 281(7), 1901-1908. https://doi.org/10.1016/j.optcom.2007.04.069

Enhanced biological cathodoluminescence. / Fisher, Phyllis J.; Wessels, William S.; Dietz, Allan B; Prendergast, Franklyn G.

In: Optics Communications, Vol. 281, No. 7, 01.04.2008, p. 1901-1908.

Research output: Contribution to journalArticle

Fisher, PJ, Wessels, WS, Dietz, AB & Prendergast, FG 2008, 'Enhanced biological cathodoluminescence', Optics Communications, vol. 281, no. 7, pp. 1901-1908. https://doi.org/10.1016/j.optcom.2007.04.069
Fisher, Phyllis J. ; Wessels, William S. ; Dietz, Allan B ; Prendergast, Franklyn G. / Enhanced biological cathodoluminescence. In: Optics Communications. 2008 ; Vol. 281, No. 7. pp. 1901-1908.
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