Enhanced binding of low-affinity antibodies interacting simultaneously with targeted cell surface molecules and Fc receptor

Y. V. Mendez-Fernandez, R. P. Pogulis, Matthew S Block, Aaron J. Johnson, S. T. Kuhns, K. S. Allen, M. J. Hansen, Larry R Pease

Research output: Contribution to journalArticle

Abstract

The possibility that different forms of class I molecules might be expressed on the cell surface of lymphocytes has been investigated periodically over the past several decades. A series of major histocompatibility complex (MHC) class I-specific monoclonal antibodies, including the commonly used antibodies 64-3-7 and 25-D1.16, bind B cells differentially, suggesting the existence of differentially expressed class I-associated cell surface determinants on B lymphocytes. However, the ability of antibodies to bind cells is determined by the sum of interactions between the antibodies and the molecules expressed on the cell surface. The interactions of class I-specific antibodies with B cells were dissected, revealing dual specificity of the antibodies for the targeted class I molecules, as well as to Fc receptors preferentially expressed by B cells. We demonstrate that antibodies simultaneously bind targeted class I molecules and Fc receptors expressed on the surface of B cells. Simultaneous binding to two cell surface structures significantly enhances the class I-specific binding pattern of certain antibodies by increasing their avidity, leading to apparent cell-specific differences in MHC expression patterns. We conclude that no differences in MHC structures need be postulated to account for the observed binding patterns.

Original languageEnglish (US)
Pages (from-to)515-525
Number of pages11
JournalTissue Antigens
Volume60
Issue number6
DOIs
StatePublished - Dec 1 2002

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Antibody Affinity
Fc Receptors
Cell Surface Receptors
B-Lymphocytes
Antibodies
Major Histocompatibility Complex
Immunoglobulin Isotypes
Monoclonal Antibodies
Lymphocytes

Keywords

  • Antibodies
  • Antigens/peptides/epitopes
  • Fc receptors
  • Lymphocytes
  • MHC

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Enhanced binding of low-affinity antibodies interacting simultaneously with targeted cell surface molecules and Fc receptor. / Mendez-Fernandez, Y. V.; Pogulis, R. P.; Block, Matthew S; Johnson, Aaron J.; Kuhns, S. T.; Allen, K. S.; Hansen, M. J.; Pease, Larry R.

In: Tissue Antigens, Vol. 60, No. 6, 01.12.2002, p. 515-525.

Research output: Contribution to journalArticle

Mendez-Fernandez, Y. V. ; Pogulis, R. P. ; Block, Matthew S ; Johnson, Aaron J. ; Kuhns, S. T. ; Allen, K. S. ; Hansen, M. J. ; Pease, Larry R. / Enhanced binding of low-affinity antibodies interacting simultaneously with targeted cell surface molecules and Fc receptor. In: Tissue Antigens. 2002 ; Vol. 60, No. 6. pp. 515-525.
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