@inbook{3e4f40612b7542fd8a21400518440d5e,
title = "Engineering cartilage tissue by co-culturing of chondrocytes and mesenchymal stromal cells",
abstract = "Co-culture of chondrocytes and mesenchymal stromal cells (MSCs) has been shown to be beneficial in engineering cartilage tissue in vitro. In these co-cultures, MSCs increase the proliferation and matrix deposition of chondrocytes. The MSCs accomplish this beneficial effect by so-called trophic actions. Thus, large cartilage constructs can be made with a relatively small number of chondrocytes. In this chapter, we describe different methods for making co-cultures of MSCs and chondrocytes. We also provide detailed protocols for analyzing MSC-chondrocyte co-cultures with cell tracking, proliferation assays, species-specific polymerase chain reactions (PCR), rheological analysis, compression analysis, RNA-sequencing analysis, short tandem repeats analysis, and biochemical examination.",
keywords = "Cartilage engineering, Chondrocytes, Co-culture, Matrix deposition, Mesenchymal stromal cells, Trophic effects",
author = "Yao Fu and Paggi, {Carlo A.} and Amel Dudakovic and {van Wijnen}, {Andre J.} and Post, {Janine N.} and Marcel Karperien",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2021.",
year = "2021",
doi = "10.1007/978-1-0716-0989-7_5",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "53--70",
booktitle = "Methods in Molecular Biology",
}