Engineered tendon with decellularized xenotendon slices and bone marrow stromal cells: An in vivo animal study

Hiromichi Omae, Yu Long Sun, Kai Nan An, Peter C Amadio, Chunfeng D Zhao

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The purpose of this study was to investigate an engineered composite of multilayer acellular tendon slices seeded with bone marrow stromal cells (BMSCs) as a possible solution for tendon reconstruction. BMSCs were harvested from 15 rabbits and infraspinatus tendons were harvested from 17 dogs. The decellularized tendons were sectioned in longitudinal slices with a thickness of 50 μm. The BMSCs were seeded on the slices and then the slices were bundled into one composite. The composite was implanted into a rabbit patellar tendon defect. Tendon slices without BMSCs were implanted into the contralateral patellar tendon as a control. The composites were evaluated by histology and qRT-PCR. The viability of BMSCs was assessed using a fluorescent marker. Histology showed viable cells between the collagen fibres on the cell-seeded side. Analysis by qRT-PCR showed higher tenomodulin, collagen type III, MMP3 and MMP13 expressions and lower collagen type I expression in the cell-seeded composite than in the tendon slices without BMSCs. We conclude that BMSCs can survive in a multilayer composite, express a tendon phenotype and enhance the metabolism of tendon in vivo. This in vivo study suggests a potential utility of this composite in tendon reconstruction.

Original languageEnglish (US)
Pages (from-to)238-244
Number of pages7
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume6
Issue number3
DOIs
StatePublished - Mar 2012

Fingerprint

Tendons
Mesenchymal Stromal Cells
Bone
Animals
Composite materials
Patellar Ligament
Collagen
Histology
Rabbits
Multilayers
Polymerase Chain Reaction
Collagen Type III
Rotator Cuff
Collagen Type I
Metabolism
Dogs
Phenotype
Defects

Keywords

  • Bone marrow stromal cells
  • Cell tracking
  • Gene expression
  • Regeneration
  • Tendon
  • Tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

Engineered tendon with decellularized xenotendon slices and bone marrow stromal cells : An in vivo animal study. / Omae, Hiromichi; Sun, Yu Long; An, Kai Nan; Amadio, Peter C; Zhao, Chunfeng D.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 6, No. 3, 03.2012, p. 238-244.

Research output: Contribution to journalArticle

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