Endotoxin in vivo impairs endothelium-dependent relaxation of canine arteries in vitro

M. E. Wylam, R. W. Samsel, J. G. Umans, R. W. Mitchell, A. R. Leff, P. T. Schumacker

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

We studied the effect of endotoxin administration in vivo on arterial smooth muscle responses in vitro, testing the hypotheses that endotoxin augments adrenergic vasoconstriction and impairs endothelium-dependent vascular relaxation. Ten mongrel dogs were anesthetized and mechanically ventilated. Five received a bolus infusion of Escherichia coli endotoxin (5 mg/kg), and the remainder received a sham infusion. After 4 to 5 h, the anesthetized dogs were rapidly exsanguinated, and femoral, renal, and superior mesenteric arteries were removed. Arterial rings were mounted on force transducers in organ baths; contraction to phenylephrine or potassium-substituted Krebs-Henseleit solution (KCl), and relaxation to nitroprusside or acetylcholine were studied. Smooth muscle contractions to phenylephrine or KCl were similar between sham and endotoxin for each agonist. Also, nitroprusside-elicited relaxation from half-maximal phenylephrine-elicited contraction was similar. However, relaxation elicited by acetylcholine was markedly impaired in vessels from endotoxin-treated dogs. The negative log molar concentration of acetylcholine producing 50% relaxation for femoral arteries was 7.38 ± 0.11 (endotoxin) versus 8.09 ± 0.12 (control, p = 0.002), for renal arteries it was 6.71 ± 0.33 (endotoxin) versus 7.81 ± 0.18 (control, p = 0.019), and for mesenteric arteries it was 7.27 ± 0.03 (endotoxin) versus 7.95 ± 0.15 (control, p = 0.002). These results demonstrate that endotoxin treatment impairs endothelium-dependent relaxation of canine arteries in vitro. The data suggest that vascular changes in endotoxemia are accompanied by alteration in endothelial cell function, perhaps through altered endothelial production of vasodilatory mediators.

Original languageEnglish (US)
Pages (from-to)1263-1267
Number of pages5
JournalAmerican Review of Respiratory Disease
Volume142
Issue number6
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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