Endothelium-dependent responses in coronary arteries are changed with puberty in male pigs

Ritu Chatrath, Karen L. Ronningen, Sandra R. Severson, Peter LaBreche, Muthuvel Jayachandran, Margarita P. Bracamonte, Virginia M. Miller

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32 Scopus citations

Abstract

In humans, cardiovascular disease begins in young adulthood and is more prevalent in males than females. However, little is known about vascular function during transition to adulthood in males. The aim of this study was to define changes in production of endothelium-derived nitric oxide (NO) and coronary arterial responses during puberty. Plasma was collected from juvenile (2-3 mo of age) and adult (5-6 mo of age) male pigs (n = 8/group) for measurement of NO, and aortic endothelial cells were collected for measurement of mRNA and protein for endothelial NO synthase (eNOS). Although plasma NO was higher in juvenile (67.0 ± 25.6 μM) than in adult (15.0 ± 7.1 μM) male pigs, eNOS protein was similar in both groups. However, levels of mRNA for eNOS were lower in aortic endothelial cells from juvenile pigs. In rings of coronary arteries suspended in organ chambers for measurement of isometric force and contracted with PGF, relaxations to an α2-adrenergic agonist were significantly inhibited by indomethacin only in juvenile pigs [EC50 (-log M), 6.7 ± 0.3 with indomethacin and 7.7 ± 0.3 under control conditions]. N G-monomethyl-L-arginine (L-NMMA) inhibited relaxations in both groups. On the contrary, in the presence of indomethacin, relaxations to bradykinin were inhibited by L-NMMA only in arteries from adult pigs [EC 50 (-log M), 8.9 ± 0.3 with indomethacin and 8.6 ± 0.3 with addition of L-NMMA]. These results suggest that hormonal changes associated with sexual maturity may affect posttranscriptional and/or translational regulation of eNOS protein and result in lower plasma NO in adult male pigs. In addition, endothelium-derived inhibitory cyclooxygenase products seem to predominate in juveniles.

Original languageEnglish (US)
Pages (from-to)H1168-H1176
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume285
Issue number3 54-3
StatePublished - Sep 1 2003

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Keywords

  • Cyclooxygenase
  • Estrogen
  • Nitric oxide
  • Prostacyclin
  • Testosterone

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Chatrath, R., Ronningen, K. L., Severson, S. R., LaBreche, P., Jayachandran, M., Bracamonte, M. P., & Miller, V. M. (2003). Endothelium-dependent responses in coronary arteries are changed with puberty in male pigs. American Journal of Physiology - Heart and Circulatory Physiology, 285(3 54-3), H1168-H1176.