TY - JOUR
T1 - Endothelium-dependent relaxation to poly-L-arginine in canine coronary arteries
AU - Rodrigues, Alfredo J.
AU - Evora, Paulo R.B.
AU - Pearson, Paul J.
AU - Schaff, Hartzell V.
N1 - Funding Information:
This work was supported by Mayo Foundation, Rochester, NY, USA, and in part by FAPESP (Fundac¸ão de Amparo a Pesquisa do Estado de São Paulo), Brazil.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003
Y1 - 2003
N2 - The endothelium-dependent relaxation elicited by poly-L-arginine was investigated in canine coronary arteries. Poly-L-arginine (10-11 to 10-7 M) induced concentration-dependent endothelium-dependent relaxation in segments of canine coronary arteries incubated with indomethacin. In the presence of indomethacin, arteries contracted with prostaglandin F2α and exposed also to ouabain, oxyhemoglobin, or Nω-nitro-L-arginine, as well as those contracted with 20 mM of KCl, presented reduced endothelium-dependent relaxation to poly-L-arginine. The combination of Nω-nitro-L-arginine with indomethacin produced further reduction of endothelium-dependent relaxation in arteries contracted with prostaglandin F2α as well as in arteries contracted with 20 mM of KCl. However, only quinacrine or calmidazolium, both in presence of Nω-nitro-L-arginine plus indomethacin, abolished the endothelium-dependent relaxation elicited by poly-L-arginine. The relaxation to poly-L-arginine was not significantly affected by glybenclamide. The present study demonstrates that in addition to the prostacyclin and nitric oxide-dependent mechanisms, poly-L-arginine also elicits endothelium-dependent relaxation in canine coronary arteries, stimulating the release of endothelial relaxing factor (s) produced by the phospholipase A2 pathway, but independent of the cyclooxygenase pathway, which may cause hyperpolarization of the vascular smooth muscle.
AB - The endothelium-dependent relaxation elicited by poly-L-arginine was investigated in canine coronary arteries. Poly-L-arginine (10-11 to 10-7 M) induced concentration-dependent endothelium-dependent relaxation in segments of canine coronary arteries incubated with indomethacin. In the presence of indomethacin, arteries contracted with prostaglandin F2α and exposed also to ouabain, oxyhemoglobin, or Nω-nitro-L-arginine, as well as those contracted with 20 mM of KCl, presented reduced endothelium-dependent relaxation to poly-L-arginine. The combination of Nω-nitro-L-arginine with indomethacin produced further reduction of endothelium-dependent relaxation in arteries contracted with prostaglandin F2α as well as in arteries contracted with 20 mM of KCl. However, only quinacrine or calmidazolium, both in presence of Nω-nitro-L-arginine plus indomethacin, abolished the endothelium-dependent relaxation elicited by poly-L-arginine. The relaxation to poly-L-arginine was not significantly affected by glybenclamide. The present study demonstrates that in addition to the prostacyclin and nitric oxide-dependent mechanisms, poly-L-arginine also elicits endothelium-dependent relaxation in canine coronary arteries, stimulating the release of endothelial relaxing factor (s) produced by the phospholipase A2 pathway, but independent of the cyclooxygenase pathway, which may cause hyperpolarization of the vascular smooth muscle.
KW - Cationic proteins
KW - Coronary artery
KW - EDHF
KW - EDRF
KW - Endothelium-dependent relaxation
KW - Endothelium-derived hyperpolarizing factor
KW - Poly-L-arginine
KW - Polycations
UR - http://www.scopus.com/inward/record.url?scp=0041564108&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041564108&partnerID=8YFLogxK
U2 - 10.1080/10623320303367
DO - 10.1080/10623320303367
M3 - Article
C2 - 12791515
AN - SCOPUS:0041564108
SN - 1062-3329
VL - 10
SP - 79
EP - 87
JO - Endothelium: Journal of Endothelial Cell Research
JF - Endothelium: Journal of Endothelial Cell Research
IS - 2
ER -