Endothelin induces vasoconstriction in the bone vasculature in vitro: An effect mediated by a single receptor population

B. C. Coessens, Virginia M Miller, M. B. Wood

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The aim of this study was to define the types of endothelin receptors present in the canine tibial vasculature. Endothelin receptor agonists and antagonists were used in two different models: isolated nutrient tibial arteries in organ bath and in vitro-perfused canine tibial bones. In isolated nutrient tibial arteries, endothelin-1 caused concentration-dependent contractions of rings with and without endothelium. BQ-123, a selective endothelin-A antagonist, induced a significant rightward shift of endothelin- 1 concentration response curves. No contractions were observed with sarafotoxin S6c, a selective endothelin-B agonist. The responses of endothelin-1 were not affected by the presence of N(G) monomethyl-L-arginine acetate plus indomethacin or by removal of the endothelium. In perfused tibial bones, endothelin-1 was more potent than endothelin-3 in causing concentration-dependent contractions. Neither endothelin-1, endothelin-3, nor sarafotoxin S6c caused relaxations. Neither the inhibition of nitric oxide nor the inhibition of prostaglandins significantly altered contractions to endothelin-1. These concordant data indicate that endothelin is a vasoconstrictor in the bone vasculature, an effect that appears to be mediated only through endothelin-A receptors.

Original languageEnglish (US)
Pages (from-to)611-617
Number of pages7
JournalJournal of Orthopaedic Research
Volume14
Issue number4
StatePublished - Jul 1996

Fingerprint

Endothelins
Endothelin-1
Vasoconstriction
Bone and Bones
Endothelin-3
Tibial Arteries
Population
Endothelium
Canidae
Endothelin A Receptors
Food
Endothelin Receptors
Vasoconstrictor Agents
Baths
Indomethacin
Prostaglandins
Arginine
In Vitro Techniques
Nitric Oxide
Acetates

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Endothelin induces vasoconstriction in the bone vasculature in vitro : An effect mediated by a single receptor population. / Coessens, B. C.; Miller, Virginia M; Wood, M. B.

In: Journal of Orthopaedic Research, Vol. 14, No. 4, 07.1996, p. 611-617.

Research output: Contribution to journalArticle

@article{75192a5e299d4113a5e1c2156390a61b,
title = "Endothelin induces vasoconstriction in the bone vasculature in vitro: An effect mediated by a single receptor population",
abstract = "The aim of this study was to define the types of endothelin receptors present in the canine tibial vasculature. Endothelin receptor agonists and antagonists were used in two different models: isolated nutrient tibial arteries in organ bath and in vitro-perfused canine tibial bones. In isolated nutrient tibial arteries, endothelin-1 caused concentration-dependent contractions of rings with and without endothelium. BQ-123, a selective endothelin-A antagonist, induced a significant rightward shift of endothelin- 1 concentration response curves. No contractions were observed with sarafotoxin S6c, a selective endothelin-B agonist. The responses of endothelin-1 were not affected by the presence of N(G) monomethyl-L-arginine acetate plus indomethacin or by removal of the endothelium. In perfused tibial bones, endothelin-1 was more potent than endothelin-3 in causing concentration-dependent contractions. Neither endothelin-1, endothelin-3, nor sarafotoxin S6c caused relaxations. Neither the inhibition of nitric oxide nor the inhibition of prostaglandins significantly altered contractions to endothelin-1. These concordant data indicate that endothelin is a vasoconstrictor in the bone vasculature, an effect that appears to be mediated only through endothelin-A receptors.",
author = "Coessens, {B. C.} and Miller, {Virginia M} and Wood, {M. B.}",
year = "1996",
month = "7",
language = "English (US)",
volume = "14",
pages = "611--617",
journal = "Journal of Orthopaedic Research",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

TY - JOUR

T1 - Endothelin induces vasoconstriction in the bone vasculature in vitro

T2 - An effect mediated by a single receptor population

AU - Coessens, B. C.

AU - Miller, Virginia M

AU - Wood, M. B.

PY - 1996/7

Y1 - 1996/7

N2 - The aim of this study was to define the types of endothelin receptors present in the canine tibial vasculature. Endothelin receptor agonists and antagonists were used in two different models: isolated nutrient tibial arteries in organ bath and in vitro-perfused canine tibial bones. In isolated nutrient tibial arteries, endothelin-1 caused concentration-dependent contractions of rings with and without endothelium. BQ-123, a selective endothelin-A antagonist, induced a significant rightward shift of endothelin- 1 concentration response curves. No contractions were observed with sarafotoxin S6c, a selective endothelin-B agonist. The responses of endothelin-1 were not affected by the presence of N(G) monomethyl-L-arginine acetate plus indomethacin or by removal of the endothelium. In perfused tibial bones, endothelin-1 was more potent than endothelin-3 in causing concentration-dependent contractions. Neither endothelin-1, endothelin-3, nor sarafotoxin S6c caused relaxations. Neither the inhibition of nitric oxide nor the inhibition of prostaglandins significantly altered contractions to endothelin-1. These concordant data indicate that endothelin is a vasoconstrictor in the bone vasculature, an effect that appears to be mediated only through endothelin-A receptors.

AB - The aim of this study was to define the types of endothelin receptors present in the canine tibial vasculature. Endothelin receptor agonists and antagonists were used in two different models: isolated nutrient tibial arteries in organ bath and in vitro-perfused canine tibial bones. In isolated nutrient tibial arteries, endothelin-1 caused concentration-dependent contractions of rings with and without endothelium. BQ-123, a selective endothelin-A antagonist, induced a significant rightward shift of endothelin- 1 concentration response curves. No contractions were observed with sarafotoxin S6c, a selective endothelin-B agonist. The responses of endothelin-1 were not affected by the presence of N(G) monomethyl-L-arginine acetate plus indomethacin or by removal of the endothelium. In perfused tibial bones, endothelin-1 was more potent than endothelin-3 in causing concentration-dependent contractions. Neither endothelin-1, endothelin-3, nor sarafotoxin S6c caused relaxations. Neither the inhibition of nitric oxide nor the inhibition of prostaglandins significantly altered contractions to endothelin-1. These concordant data indicate that endothelin is a vasoconstrictor in the bone vasculature, an effect that appears to be mediated only through endothelin-A receptors.

UR - http://www.scopus.com/inward/record.url?scp=0030198636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030198636&partnerID=8YFLogxK

M3 - Article

C2 - 8764871

AN - SCOPUS:0030198636

VL - 14

SP - 611

EP - 617

JO - Journal of Orthopaedic Research

JF - Journal of Orthopaedic Research

SN - 0736-0266

IS - 4

ER -