Endothelialization of magnetic graft materials using SPION-labeled endothelial cells

Brant R. Newman, Dan Dragomir-Daescu, Adriana Harbuzariu, Malcolm McIntosh, J. Jonathan Harburn, Anthony Parakka, Manju Kalra, David Holmes, Robert D. Simari, Gurpreet S. Sandhu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Seeding vascular grafts with autologous endothelial cells (EC) has been shown to improve in vivo patency, but high cost and development time have prevented widespread clinical use. A technique for loading EC with superparamagnetic iron-oxide nanospheres (SPIONs) was recently described. SPION-loaded EC experience magnetic attractive forces in the presence of sufficient magnetic field gradients. Using a multi-factorial design of experiments approach, the quantity and spatial distribution of magnetizable metal particles within a poly (ether urethane) matrix were systematically varied to produce unique material specimens. Specimens were seeded with SPION-loaded ECs, and cell coverage was quantified at various post-seeding time intervals using micrographic image analysis. The effects of changing design parameters on cell capture and sustained cell viability on magnetic substrates were statistically examined. Magnetized ferrites and samarium cobalt demonstrated cell capture, though cytotoxicity prevented sustained cell growth. Cobalt chromium substrates showed effective cell capture and growth to near complete confluence for up to one month.

Original languageEnglish (US)
Title of host publication8th International Conference on the Scientific and Clinical Applications of Magnetic Carriers
Pages395-400
Number of pages6
DOIs
StatePublished - 2010
Event8th International Conference on the Scientific and Clinical Applications of Magnetic Carriers - Rostock, Germany
Duration: May 25 2010May 29 2010

Publication series

NameAIP Conference Proceedings
Volume1311
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other8th International Conference on the Scientific and Clinical Applications of Magnetic Carriers
CountryGermany
CityRostock
Period5/25/105/29/10

Keywords

  • Cell seeding
  • Cobalt chromium
  • SPION
  • Vascular graft

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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  • Cite this

    Newman, B. R., Dragomir-Daescu, D., Harbuzariu, A., McIntosh, M., Harburn, J. J., Parakka, A., Kalra, M., Holmes, D., Simari, R. D., & Sandhu, G. S. (2010). Endothelialization of magnetic graft materials using SPION-labeled endothelial cells. In 8th International Conference on the Scientific and Clinical Applications of Magnetic Carriers (pp. 395-400). (AIP Conference Proceedings; Vol. 1311). https://doi.org/10.1063/1.3530044