Endothelial sodding of the Permaflow prosthetic coronary artery bypass conduit

Michael R. Phillips, Hiroki Yamaguchi, Virginia M Miller, Stuart Williams, James J. Morris, Hartzell V Schaff

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background. Experiments were designed to determine the feasibility of sodding an endothelial monolayer within the lumen of a prosthetic conduit applied to the canine coronary circulation. Methods. Autologous endothelial cells were sodded onto the luminal surface of the Permaflow conduit and immediately implanted to bypass the left circumflex coronary artery in adult mongrel dogs (n = 9). Unsodded Permaflow conduits were implanted as controls (n = 8). At 3 weeks, grafts were explanted and examined by scanning electron microscopy and immunostained for canine von Willebrand factor. Results. Sodded grafts contained a confluent endothelial cell layer devoid of adherent thrombus or platelets and stained positively for canine von Willebrand factor. Unsodded grafts contained no endothelium and retained adherent platelets, collagen, and fibrin. Effluent from sodded grafts stimulated with calcium ionophore A23187 caused a significantly greater relaxation of its bioassay ring than effluent from unsodded grafts (60% ± 21% versus 12% ± 5%; n = 8, p < 0.03). Conclusions. Sodding of endothelial cells onto a Permaflow coronary artery bypass graft results in a confluent, viable, nonthrombogenic, endothelial monolayer and releases vasodilator substances in response to calcium ionophore A23187. Endothelial sodding may optimize prosthetic grafts.

Original languageEnglish (US)
Pages (from-to)1191-1197
Number of pages7
JournalAnnals of Thoracic Surgery
Volume66
Issue number4
DOIs
StatePublished - Oct 1998

Fingerprint

Coronary Artery Bypass
Transplants
Canidae
Endothelial Cells
Calcium Ionophores
Calcimycin
von Willebrand Factor
Blood Platelets
Coronary Circulation
PermaFlow
Fibrin
Vasodilator Agents
Biological Assay
Electron Scanning Microscopy
Endothelium
Coronary Vessels
Thrombosis
Collagen
Dogs

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Endothelial sodding of the Permaflow prosthetic coronary artery bypass conduit. / Phillips, Michael R.; Yamaguchi, Hiroki; Miller, Virginia M; Williams, Stuart; Morris, James J.; Schaff, Hartzell V.

In: Annals of Thoracic Surgery, Vol. 66, No. 4, 10.1998, p. 1191-1197.

Research output: Contribution to journalArticle

Phillips, Michael R. ; Yamaguchi, Hiroki ; Miller, Virginia M ; Williams, Stuart ; Morris, James J. ; Schaff, Hartzell V. / Endothelial sodding of the Permaflow prosthetic coronary artery bypass conduit. In: Annals of Thoracic Surgery. 1998 ; Vol. 66, No. 4. pp. 1191-1197.
@article{a894988ce4e34fda84cdc2be6262b8d8,
title = "Endothelial sodding of the Permaflow prosthetic coronary artery bypass conduit",
abstract = "Background. Experiments were designed to determine the feasibility of sodding an endothelial monolayer within the lumen of a prosthetic conduit applied to the canine coronary circulation. Methods. Autologous endothelial cells were sodded onto the luminal surface of the Permaflow conduit and immediately implanted to bypass the left circumflex coronary artery in adult mongrel dogs (n = 9). Unsodded Permaflow conduits were implanted as controls (n = 8). At 3 weeks, grafts were explanted and examined by scanning electron microscopy and immunostained for canine von Willebrand factor. Results. Sodded grafts contained a confluent endothelial cell layer devoid of adherent thrombus or platelets and stained positively for canine von Willebrand factor. Unsodded grafts contained no endothelium and retained adherent platelets, collagen, and fibrin. Effluent from sodded grafts stimulated with calcium ionophore A23187 caused a significantly greater relaxation of its bioassay ring than effluent from unsodded grafts (60{\%} ± 21{\%} versus 12{\%} ± 5{\%}; n = 8, p < 0.03). Conclusions. Sodding of endothelial cells onto a Permaflow coronary artery bypass graft results in a confluent, viable, nonthrombogenic, endothelial monolayer and releases vasodilator substances in response to calcium ionophore A23187. Endothelial sodding may optimize prosthetic grafts.",
author = "Phillips, {Michael R.} and Hiroki Yamaguchi and Miller, {Virginia M} and Stuart Williams and Morris, {James J.} and Schaff, {Hartzell V}",
year = "1998",
month = "10",
doi = "10.1016/S0003-4975(98)00780-2",
language = "English (US)",
volume = "66",
pages = "1191--1197",
journal = "Annals of Thoracic Surgery",
issn = "0003-4975",
publisher = "Elsevier USA",
number = "4",

}

TY - JOUR

T1 - Endothelial sodding of the Permaflow prosthetic coronary artery bypass conduit

AU - Phillips, Michael R.

AU - Yamaguchi, Hiroki

AU - Miller, Virginia M

AU - Williams, Stuart

AU - Morris, James J.

AU - Schaff, Hartzell V

PY - 1998/10

Y1 - 1998/10

N2 - Background. Experiments were designed to determine the feasibility of sodding an endothelial monolayer within the lumen of a prosthetic conduit applied to the canine coronary circulation. Methods. Autologous endothelial cells were sodded onto the luminal surface of the Permaflow conduit and immediately implanted to bypass the left circumflex coronary artery in adult mongrel dogs (n = 9). Unsodded Permaflow conduits were implanted as controls (n = 8). At 3 weeks, grafts were explanted and examined by scanning electron microscopy and immunostained for canine von Willebrand factor. Results. Sodded grafts contained a confluent endothelial cell layer devoid of adherent thrombus or platelets and stained positively for canine von Willebrand factor. Unsodded grafts contained no endothelium and retained adherent platelets, collagen, and fibrin. Effluent from sodded grafts stimulated with calcium ionophore A23187 caused a significantly greater relaxation of its bioassay ring than effluent from unsodded grafts (60% ± 21% versus 12% ± 5%; n = 8, p < 0.03). Conclusions. Sodding of endothelial cells onto a Permaflow coronary artery bypass graft results in a confluent, viable, nonthrombogenic, endothelial monolayer and releases vasodilator substances in response to calcium ionophore A23187. Endothelial sodding may optimize prosthetic grafts.

AB - Background. Experiments were designed to determine the feasibility of sodding an endothelial monolayer within the lumen of a prosthetic conduit applied to the canine coronary circulation. Methods. Autologous endothelial cells were sodded onto the luminal surface of the Permaflow conduit and immediately implanted to bypass the left circumflex coronary artery in adult mongrel dogs (n = 9). Unsodded Permaflow conduits were implanted as controls (n = 8). At 3 weeks, grafts were explanted and examined by scanning electron microscopy and immunostained for canine von Willebrand factor. Results. Sodded grafts contained a confluent endothelial cell layer devoid of adherent thrombus or platelets and stained positively for canine von Willebrand factor. Unsodded grafts contained no endothelium and retained adherent platelets, collagen, and fibrin. Effluent from sodded grafts stimulated with calcium ionophore A23187 caused a significantly greater relaxation of its bioassay ring than effluent from unsodded grafts (60% ± 21% versus 12% ± 5%; n = 8, p < 0.03). Conclusions. Sodding of endothelial cells onto a Permaflow coronary artery bypass graft results in a confluent, viable, nonthrombogenic, endothelial monolayer and releases vasodilator substances in response to calcium ionophore A23187. Endothelial sodding may optimize prosthetic grafts.

UR - http://www.scopus.com/inward/record.url?scp=0032191967&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032191967&partnerID=8YFLogxK

U2 - 10.1016/S0003-4975(98)00780-2

DO - 10.1016/S0003-4975(98)00780-2

M3 - Article

VL - 66

SP - 1191

EP - 1197

JO - Annals of Thoracic Surgery

JF - Annals of Thoracic Surgery

SN - 0003-4975

IS - 4

ER -