Endothelial regulation of vascular contraction in radial and internal mammary arteries

David G. Cable, James A. Caccitolo, Eric A. Pfeifer, Richard C. Daly, Joseph A. Dearani, Charles J. Mullany, Timothy O'Brien, Thomas A. Orszulak, Hartzell V Schaff

Research output: Contribution to journalArticle

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Abstract

Background. The extent to which the endothelium regulates radial artery (RA) contractions is unknown. The goals of this study were to characterize endothelium-dependent relaxations in the RA, compare these responses with those in the internal mammary artery (IMA), and, subsequently, manipulate nitric oxide production in the RA with adenovirus-mediated gene transfer. Methods. Segments of RA and IMA from 43 patients were studied initially in organ chambers. Endothelial function was evaluated and gene transfer, was examined. Results. After precontraction to 80% maximum tension with prostaglandin F(2α), acetylcholine produced lesser relaxations in the RA (21.5% ± 5.8%) than in the IMA (66.7% ± 10.6%); human thrombin and adenosine 5'-diphosphate yielded similar results. Reduced relaxations in the RA (16.8% ± 4.2%) compared with those in the IMA (71.6% ± 11.9%) were noted with calcium ionophore. Superfusion bioassay demonstrated a similar baseline release in both arteries but a reduced stimulated production of vasoactive substances in the RA, results confirmed by cyclic guanosine monophosphate level determination. The RA produced less 6-keto-prostaglandin F(1α) than the IMA. Light microscopy demonstrated an intact endothelium in both arteries. Adenovirus-mediated gene transfer of nitric oxide synthase augmented relaxations of the RA to acetylcholine. Conclusions. Reduced production of endothelium-derived relaxing factors suggests diminished endothelial regulation of vascular smooth muscle in the RA compared with the IMA. This finding may explain, in part, the predisposition to vasoconstriction in RA grafts.

Original languageEnglish (US)
Pages (from-to)1083-1090
Number of pages8
JournalAnnals of Thoracic Surgery
Volume67
Issue number4
DOIs
StatePublished - Apr 1999

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Radial Artery
Mammary Arteries
Blood Vessels
Endothelium
Prostaglandins F
Adenoviridae
Acetylcholine
Arteries
Genes
Endothelium-Dependent Relaxing Factors
Calcium Ionophores
Cyclic GMP
Vasoconstriction
Vascular Smooth Muscle
Nitric Oxide Synthase
Thrombin
Biological Assay
Adenosine Diphosphate
Microscopy
Nitric Oxide

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Cable, D. G., Caccitolo, J. A., Pfeifer, E. A., Daly, R. C., Dearani, J. A., Mullany, C. J., ... Schaff, H. V. (1999). Endothelial regulation of vascular contraction in radial and internal mammary arteries. Annals of Thoracic Surgery, 67(4), 1083-1090. https://doi.org/10.1016/S0003-4975(99)00130-7

Endothelial regulation of vascular contraction in radial and internal mammary arteries. / Cable, David G.; Caccitolo, James A.; Pfeifer, Eric A.; Daly, Richard C.; Dearani, Joseph A.; Mullany, Charles J.; O'Brien, Timothy; Orszulak, Thomas A.; Schaff, Hartzell V.

In: Annals of Thoracic Surgery, Vol. 67, No. 4, 04.1999, p. 1083-1090.

Research output: Contribution to journalArticle

Cable, DG, Caccitolo, JA, Pfeifer, EA, Daly, RC, Dearani, JA, Mullany, CJ, O'Brien, T, Orszulak, TA & Schaff, HV 1999, 'Endothelial regulation of vascular contraction in radial and internal mammary arteries', Annals of Thoracic Surgery, vol. 67, no. 4, pp. 1083-1090. https://doi.org/10.1016/S0003-4975(99)00130-7
Cable DG, Caccitolo JA, Pfeifer EA, Daly RC, Dearani JA, Mullany CJ et al. Endothelial regulation of vascular contraction in radial and internal mammary arteries. Annals of Thoracic Surgery. 1999 Apr;67(4):1083-1090. https://doi.org/10.1016/S0003-4975(99)00130-7
Cable, David G. ; Caccitolo, James A. ; Pfeifer, Eric A. ; Daly, Richard C. ; Dearani, Joseph A. ; Mullany, Charles J. ; O'Brien, Timothy ; Orszulak, Thomas A. ; Schaff, Hartzell V. / Endothelial regulation of vascular contraction in radial and internal mammary arteries. In: Annals of Thoracic Surgery. 1999 ; Vol. 67, No. 4. pp. 1083-1090.
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abstract = "Background. The extent to which the endothelium regulates radial artery (RA) contractions is unknown. The goals of this study were to characterize endothelium-dependent relaxations in the RA, compare these responses with those in the internal mammary artery (IMA), and, subsequently, manipulate nitric oxide production in the RA with adenovirus-mediated gene transfer. Methods. Segments of RA and IMA from 43 patients were studied initially in organ chambers. Endothelial function was evaluated and gene transfer, was examined. Results. After precontraction to 80{\%} maximum tension with prostaglandin F(2α), acetylcholine produced lesser relaxations in the RA (21.5{\%} ± 5.8{\%}) than in the IMA (66.7{\%} ± 10.6{\%}); human thrombin and adenosine 5'-diphosphate yielded similar results. Reduced relaxations in the RA (16.8{\%} ± 4.2{\%}) compared with those in the IMA (71.6{\%} ± 11.9{\%}) were noted with calcium ionophore. Superfusion bioassay demonstrated a similar baseline release in both arteries but a reduced stimulated production of vasoactive substances in the RA, results confirmed by cyclic guanosine monophosphate level determination. The RA produced less 6-keto-prostaglandin F(1α) than the IMA. Light microscopy demonstrated an intact endothelium in both arteries. Adenovirus-mediated gene transfer of nitric oxide synthase augmented relaxations of the RA to acetylcholine. Conclusions. Reduced production of endothelium-derived relaxing factors suggests diminished endothelial regulation of vascular smooth muscle in the RA compared with the IMA. This finding may explain, in part, the predisposition to vasoconstriction in RA grafts.",
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AU - Cable, David G.

AU - Caccitolo, James A.

AU - Pfeifer, Eric A.

AU - Daly, Richard C.

AU - Dearani, Joseph A.

AU - Mullany, Charles J.

AU - O'Brien, Timothy

AU - Orszulak, Thomas A.

AU - Schaff, Hartzell V

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N2 - Background. The extent to which the endothelium regulates radial artery (RA) contractions is unknown. The goals of this study were to characterize endothelium-dependent relaxations in the RA, compare these responses with those in the internal mammary artery (IMA), and, subsequently, manipulate nitric oxide production in the RA with adenovirus-mediated gene transfer. Methods. Segments of RA and IMA from 43 patients were studied initially in organ chambers. Endothelial function was evaluated and gene transfer, was examined. Results. After precontraction to 80% maximum tension with prostaglandin F(2α), acetylcholine produced lesser relaxations in the RA (21.5% ± 5.8%) than in the IMA (66.7% ± 10.6%); human thrombin and adenosine 5'-diphosphate yielded similar results. Reduced relaxations in the RA (16.8% ± 4.2%) compared with those in the IMA (71.6% ± 11.9%) were noted with calcium ionophore. Superfusion bioassay demonstrated a similar baseline release in both arteries but a reduced stimulated production of vasoactive substances in the RA, results confirmed by cyclic guanosine monophosphate level determination. The RA produced less 6-keto-prostaglandin F(1α) than the IMA. Light microscopy demonstrated an intact endothelium in both arteries. Adenovirus-mediated gene transfer of nitric oxide synthase augmented relaxations of the RA to acetylcholine. Conclusions. Reduced production of endothelium-derived relaxing factors suggests diminished endothelial regulation of vascular smooth muscle in the RA compared with the IMA. This finding may explain, in part, the predisposition to vasoconstriction in RA grafts.

AB - Background. The extent to which the endothelium regulates radial artery (RA) contractions is unknown. The goals of this study were to characterize endothelium-dependent relaxations in the RA, compare these responses with those in the internal mammary artery (IMA), and, subsequently, manipulate nitric oxide production in the RA with adenovirus-mediated gene transfer. Methods. Segments of RA and IMA from 43 patients were studied initially in organ chambers. Endothelial function was evaluated and gene transfer, was examined. Results. After precontraction to 80% maximum tension with prostaglandin F(2α), acetylcholine produced lesser relaxations in the RA (21.5% ± 5.8%) than in the IMA (66.7% ± 10.6%); human thrombin and adenosine 5'-diphosphate yielded similar results. Reduced relaxations in the RA (16.8% ± 4.2%) compared with those in the IMA (71.6% ± 11.9%) were noted with calcium ionophore. Superfusion bioassay demonstrated a similar baseline release in both arteries but a reduced stimulated production of vasoactive substances in the RA, results confirmed by cyclic guanosine monophosphate level determination. The RA produced less 6-keto-prostaglandin F(1α) than the IMA. Light microscopy demonstrated an intact endothelium in both arteries. Adenovirus-mediated gene transfer of nitric oxide synthase augmented relaxations of the RA to acetylcholine. Conclusions. Reduced production of endothelium-derived relaxing factors suggests diminished endothelial regulation of vascular smooth muscle in the RA compared with the IMA. This finding may explain, in part, the predisposition to vasoconstriction in RA grafts.

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