Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity

Anjali Verma, Resham Bhattacharya, Indu Remadevi, Keguo Li, Kallal Pramanik, Ganesh V. Samant, Mark Horswill, Chang Z. Chun, Baofeng Zhao, Enfeng Wang, Robert Qing Miao, Debabrata Mukhopadhyay, Ramani Ramchandran, George A. Wilkinson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Endothelial cell - specific chemotaxis receptor (ECSCR) is a cell surface protein expressed by blood endothelial cells with roles in endothelial cell migration and signal transduction. We investigated the function of ecscr in the development of the zebrafish vasculature. Zebrafish ecscr is expressed in angioblasts and in axial vessels during angioblast migration and vasculogenesis. Morpholino-directed ecscr knockdown resulted in defective angioblast migration in the posterior lateral plate mesoderm, a process known to depend on vascular endothelial-derived growth factor (VEGF). In cultured cells, transfected ECSCR localized to actin-rich membrane protrusions, colocalizing with kinase insert domain protein receptor (KDR)/VEGF receptor 2 in these regions. ECSCR-silenced cells show reduced VEGF-induced phosphorylation of KDR but not of FMS-like tyrosine kinase 1 (FLT1)/VEGF receptor 1. Finally, chemical inhibition of VEGF receptor activity in zebrafish resulted in angioblast deficiencies that partially overlap with those seen in ecscr morphants. We propose that ecscr promotes migration of zebrafish angioblasts by enhancing endothelial kdr sensitivity to VEGF.

Original languageEnglish (US)
Pages (from-to)4614-4622
Number of pages9
JournalBlood
Volume115
Issue number22
DOIs
StatePublished - Jun 3 2010
Externally publishedYes

Fingerprint

Vascular Endothelial Growth Factor Receptor
Growth Factor Receptors
Endothelial cells
Chemotaxis
Endothelial Cells
Zebrafish
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-2
Intercellular Signaling Peptides and Proteins
Vascular Endothelial Growth Factor Receptor-1
Morpholinos
Signal transduction
Phosphorylation
Die casting inserts
Mesoderm
Protein-Tyrosine Kinases
Cell Movement
Actins
Cultured Cells
Signal Transduction

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Verma, A., Bhattacharya, R., Remadevi, I., Li, K., Pramanik, K., Samant, G. V., ... Wilkinson, G. A. (2010). Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity. Blood, 115(22), 4614-4622. https://doi.org/10.1182/blood-2009-10-248856

Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity. / Verma, Anjali; Bhattacharya, Resham; Remadevi, Indu; Li, Keguo; Pramanik, Kallal; Samant, Ganesh V.; Horswill, Mark; Chun, Chang Z.; Zhao, Baofeng; Wang, Enfeng; Miao, Robert Qing; Mukhopadhyay, Debabrata; Ramchandran, Ramani; Wilkinson, George A.

In: Blood, Vol. 115, No. 22, 03.06.2010, p. 4614-4622.

Research output: Contribution to journalArticle

Verma, A, Bhattacharya, R, Remadevi, I, Li, K, Pramanik, K, Samant, GV, Horswill, M, Chun, CZ, Zhao, B, Wang, E, Miao, RQ, Mukhopadhyay, D, Ramchandran, R & Wilkinson, GA 2010, 'Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity', Blood, vol. 115, no. 22, pp. 4614-4622. https://doi.org/10.1182/blood-2009-10-248856
Verma, Anjali ; Bhattacharya, Resham ; Remadevi, Indu ; Li, Keguo ; Pramanik, Kallal ; Samant, Ganesh V. ; Horswill, Mark ; Chun, Chang Z. ; Zhao, Baofeng ; Wang, Enfeng ; Miao, Robert Qing ; Mukhopadhyay, Debabrata ; Ramchandran, Ramani ; Wilkinson, George A. / Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity. In: Blood. 2010 ; Vol. 115, No. 22. pp. 4614-4622.
@article{6df031636e164304b71449cb86ceb4a9,
title = "Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity",
abstract = "Endothelial cell - specific chemotaxis receptor (ECSCR) is a cell surface protein expressed by blood endothelial cells with roles in endothelial cell migration and signal transduction. We investigated the function of ecscr in the development of the zebrafish vasculature. Zebrafish ecscr is expressed in angioblasts and in axial vessels during angioblast migration and vasculogenesis. Morpholino-directed ecscr knockdown resulted in defective angioblast migration in the posterior lateral plate mesoderm, a process known to depend on vascular endothelial-derived growth factor (VEGF). In cultured cells, transfected ECSCR localized to actin-rich membrane protrusions, colocalizing with kinase insert domain protein receptor (KDR)/VEGF receptor 2 in these regions. ECSCR-silenced cells show reduced VEGF-induced phosphorylation of KDR but not of FMS-like tyrosine kinase 1 (FLT1)/VEGF receptor 1. Finally, chemical inhibition of VEGF receptor activity in zebrafish resulted in angioblast deficiencies that partially overlap with those seen in ecscr morphants. We propose that ecscr promotes migration of zebrafish angioblasts by enhancing endothelial kdr sensitivity to VEGF.",
author = "Anjali Verma and Resham Bhattacharya and Indu Remadevi and Keguo Li and Kallal Pramanik and Samant, {Ganesh V.} and Mark Horswill and Chun, {Chang Z.} and Baofeng Zhao and Enfeng Wang and Miao, {Robert Qing} and Debabrata Mukhopadhyay and Ramani Ramchandran and Wilkinson, {George A.}",
year = "2010",
month = "6",
day = "3",
doi = "10.1182/blood-2009-10-248856",
language = "English (US)",
volume = "115",
pages = "4614--4622",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "22",

}

TY - JOUR

T1 - Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity

AU - Verma, Anjali

AU - Bhattacharya, Resham

AU - Remadevi, Indu

AU - Li, Keguo

AU - Pramanik, Kallal

AU - Samant, Ganesh V.

AU - Horswill, Mark

AU - Chun, Chang Z.

AU - Zhao, Baofeng

AU - Wang, Enfeng

AU - Miao, Robert Qing

AU - Mukhopadhyay, Debabrata

AU - Ramchandran, Ramani

AU - Wilkinson, George A.

PY - 2010/6/3

Y1 - 2010/6/3

N2 - Endothelial cell - specific chemotaxis receptor (ECSCR) is a cell surface protein expressed by blood endothelial cells with roles in endothelial cell migration and signal transduction. We investigated the function of ecscr in the development of the zebrafish vasculature. Zebrafish ecscr is expressed in angioblasts and in axial vessels during angioblast migration and vasculogenesis. Morpholino-directed ecscr knockdown resulted in defective angioblast migration in the posterior lateral plate mesoderm, a process known to depend on vascular endothelial-derived growth factor (VEGF). In cultured cells, transfected ECSCR localized to actin-rich membrane protrusions, colocalizing with kinase insert domain protein receptor (KDR)/VEGF receptor 2 in these regions. ECSCR-silenced cells show reduced VEGF-induced phosphorylation of KDR but not of FMS-like tyrosine kinase 1 (FLT1)/VEGF receptor 1. Finally, chemical inhibition of VEGF receptor activity in zebrafish resulted in angioblast deficiencies that partially overlap with those seen in ecscr morphants. We propose that ecscr promotes migration of zebrafish angioblasts by enhancing endothelial kdr sensitivity to VEGF.

AB - Endothelial cell - specific chemotaxis receptor (ECSCR) is a cell surface protein expressed by blood endothelial cells with roles in endothelial cell migration and signal transduction. We investigated the function of ecscr in the development of the zebrafish vasculature. Zebrafish ecscr is expressed in angioblasts and in axial vessels during angioblast migration and vasculogenesis. Morpholino-directed ecscr knockdown resulted in defective angioblast migration in the posterior lateral plate mesoderm, a process known to depend on vascular endothelial-derived growth factor (VEGF). In cultured cells, transfected ECSCR localized to actin-rich membrane protrusions, colocalizing with kinase insert domain protein receptor (KDR)/VEGF receptor 2 in these regions. ECSCR-silenced cells show reduced VEGF-induced phosphorylation of KDR but not of FMS-like tyrosine kinase 1 (FLT1)/VEGF receptor 1. Finally, chemical inhibition of VEGF receptor activity in zebrafish resulted in angioblast deficiencies that partially overlap with those seen in ecscr morphants. We propose that ecscr promotes migration of zebrafish angioblasts by enhancing endothelial kdr sensitivity to VEGF.

UR - http://www.scopus.com/inward/record.url?scp=77953922296&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953922296&partnerID=8YFLogxK

U2 - 10.1182/blood-2009-10-248856

DO - 10.1182/blood-2009-10-248856

M3 - Article

VL - 115

SP - 4614

EP - 4622

JO - Blood

JF - Blood

SN - 0006-4971

IS - 22

ER -