Endogenous tau aggregates in oligodendrocytes of rTg4510 mice induced by human P301L tau

Yan Ren, Wen Lang Lin, Laura Sanchez, Carolina Ceballos, Manuela Polydoro, Tara L. Spires-Jones, Bradley T. Hyman, Dennis W Dickson, Naruhiko Sahara

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tau belongs to the microtubule-associated family of proteins that maintain cytoskeletal structure by regulating microtubule dynamics. In certain neurodegenerative diseases termed tauopathies, tau is abnormally phosphorylated and accumulates as filamentous inclusions. Transgenic mouse models that overexpress human tau have been widely used to investigate tau pathogenesis. Although many studies have attempted to elucidate the pathological function of transgenic human tau, it remains unknown whether endogenous mouse tau is involved in disease progression. Here we generated an mTau antibody that selectively recognizes mouse and rat tau, but not human tau. In rTg4510 tau transgenic mice, we identified a higher molecular weight mouse tau (~60-kDa) in sarkosyl-insoluble fractions. mTau antibody started to recognize intracellular aggregates and thread-like structures in 4- to 6-month-old rTg4510 mice. Tau inclusions appeared earlier, being detected in 2.5-month-old rTg4510 mice with MC1 antibody. Immunoelectron microscopy confirmed the presence of filamentous aggregates of mouse tau, which were abundant in oligodendrocytes but rare in neurons. Mouse tau inclusions in oligodendrocytes were confirmed by double-labeling with an oligodendrocyte marker. Our data indicate that mouse tau has potential aggregation properties in neurons and non-neurons. The mTau antibody will be useful for investigating the role of mouse tau in mouse models of tauopathy.

Original languageEnglish (US)
Pages (from-to)589-600
Number of pages12
JournalJournal of Alzheimer's Disease
Volume38
Issue number3
DOIs
StatePublished - 2014

Fingerprint

Oligodendroglia
Tauopathies
Antibodies
Transgenic Mice
Neurons
Microtubule-Associated Proteins
Immunoelectron Microscopy
Microtubules
Neurodegenerative Diseases
Disease Progression
Molecular Weight

Keywords

  • Antibody
  • mouse tau
  • oligodendrocytes
  • tau protein
  • tauopathy
  • transgenic mice

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Geriatrics and Gerontology
  • Clinical Psychology

Cite this

Ren, Y., Lin, W. L., Sanchez, L., Ceballos, C., Polydoro, M., Spires-Jones, T. L., ... Sahara, N. (2014). Endogenous tau aggregates in oligodendrocytes of rTg4510 mice induced by human P301L tau. Journal of Alzheimer's Disease, 38(3), 589-600. https://doi.org/10.3233/JAD-130986

Endogenous tau aggregates in oligodendrocytes of rTg4510 mice induced by human P301L tau. / Ren, Yan; Lin, Wen Lang; Sanchez, Laura; Ceballos, Carolina; Polydoro, Manuela; Spires-Jones, Tara L.; Hyman, Bradley T.; Dickson, Dennis W; Sahara, Naruhiko.

In: Journal of Alzheimer's Disease, Vol. 38, No. 3, 2014, p. 589-600.

Research output: Contribution to journalArticle

Ren, Y, Lin, WL, Sanchez, L, Ceballos, C, Polydoro, M, Spires-Jones, TL, Hyman, BT, Dickson, DW & Sahara, N 2014, 'Endogenous tau aggregates in oligodendrocytes of rTg4510 mice induced by human P301L tau', Journal of Alzheimer's Disease, vol. 38, no. 3, pp. 589-600. https://doi.org/10.3233/JAD-130986
Ren, Yan ; Lin, Wen Lang ; Sanchez, Laura ; Ceballos, Carolina ; Polydoro, Manuela ; Spires-Jones, Tara L. ; Hyman, Bradley T. ; Dickson, Dennis W ; Sahara, Naruhiko. / Endogenous tau aggregates in oligodendrocytes of rTg4510 mice induced by human P301L tau. In: Journal of Alzheimer's Disease. 2014 ; Vol. 38, No. 3. pp. 589-600.
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