Endogenous immunoreactive glia maturation factor-like molecule in cultured rat Schwann cells

Ramon Lim, Danny J. Hicklin, Timothy C. Ryken, Joyce F. Miller, E. Peter Bosch

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Using the monoclonal antibody G2-09 raised against bovine glia maturation factor (GMF), we demonstrated that cultured rat Schwann cells and Schwannoma cells, but not their conditioned media, possessed endogenous GMF-like immunoreactivity. The presence of immunoreactive GMF correlated well with GMF bioactivity. The GMF-like factor in Schwann cells was characterized by immunodotting, immunofluorescence, immunoadsorption and immunoblotting. Immunofluorescence confirmed the intracellular location of GMF. Immunoadsorption completely eliminated the GMF-like bioactivity from the cell extracts. Immunoblotting identified a protein band with a molecular weight of 14,000. Thus, the evidence strongly supports the argument that the GMF-like factor in rat Schwann cells is identical with GMF from the bovine brain. The GMF-like molecule in Schwannoma cells showed properties similar to those in Schwann cells, but for unknown reasons was not detectable by immunofluorescence. The presence of GMF in cultured rat Schwann cells suggests that the factor may play a role in the peripheral nervous system.

Original languageEnglish (US)
Pages (from-to)277-284
Number of pages8
JournalDevelopmental Brain Research
Volume40
Issue number2
DOIs
StatePublished - May 16 1988
Externally publishedYes

    Fingerprint

Keywords

  • Glia maturation
  • Glia maturation factor (GMF)
  • Immunoreactivity
  • Schwann cell

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this