End-binding protein 1 controls signal propagation from the T cell receptor

Noa B. Martín-Cófreces, Francesc Baixauli, María J. López, Diana Gil, Alicia Monjas, Balbino Alarcón, Francisco Sánchez-Madrid

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The role of microtubules (MTs) in the control and dynamics of the immune synapse (IS) remains unresolved. Here, we show that T cell activation requires the growth of MTs mediated by the plus-end specific protein end-binding 1 (EB1). A direct interaction of the T cell receptor (TCR) complex with EB1 provides the molecular basis for EB1 activity promoting TCR encounter with signalling vesicles at the IS. EB1 knockdown alters TCR dynamics at the IS and prevents propagation of the TCR activation signal to LAT, thus inhibiting activation of PLCγ1 and its localization to the IS. These results identify a role for EB1 interaction with the TCR in controlling TCR sorting and its connection with the LAT/PLCγ1 signalosome.

Original languageEnglish (US)
Pages (from-to)4140-4152
Number of pages13
JournalEMBO Journal
Volume31
Issue number21
DOIs
StatePublished - Oct 31 2012

Keywords

  • T cell activation
  • cell signalling
  • cytoskeleton
  • microtubule dynamics
  • vesicular trafficking

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Martín-Cófreces, N. B., Baixauli, F., López, M. J., Gil, D., Monjas, A., Alarcón, B., & Sánchez-Madrid, F. (2012). End-binding protein 1 controls signal propagation from the T cell receptor. EMBO Journal, 31(21), 4140-4152. https://doi.org/10.1038/emboj.2012.242